苗药大乌泡茎乙醇提取物的化学成分研究

Study on Chemical Constituents in Ethanol Extract from the Stem of Miao Medicine Rubus multibracteatus

目的:研究苗药大乌泡茎乙醇提取物的化学成分。方法:采用硅胶柱、制备液相色谱、Sephadex LH-20凝胶柱等对大乌泡茎乙醇提取物进行分离纯化,根据理化性质和波谱(质谱、氢谱、碳谱)数据分析鉴定化合物结构。结果:从大乌泡茎乙醇提取物中分离得到10个化合物,分别鉴定为5,4′-二羟基-8-(3,3-二甲基烯丙基)-2″,2″-二甲基吡喃[5,6∶6,7]异黄酮(1)、3-羟基-1-(4′-羟基-3′-甲氧基苯)-1-丙酮(2)、3β-羟基豆甾-5-烯-7-酮(3)、羽扇豆醇(4)、松柏醛(5)、反式对羟基肉桂酸(6)、染料木黄酮(7)、1-O-对羟基肉桂酰单甘油酯(8)、东莨菪内酯(9)、山柰酚(10)。结论:化合物1~9均为首次从大乌泡植物中分离得到,化合物2、5、8为首次从悬钩子属植物中分离得到。该研究可为大乌泡的进一步开发利用奠定基础。

OBJECTIVE： To study the chemical constituents in ethanol extract from the stem of Miao medicine Rubus multibracteatus. METHODS：The ethanol extract from the stem of Miao medicine R. multibracteatus was isolated and purified by silica gel column,preparative liquid chromatography and Sephadex LH-20 gel column,etc. The structure of compounds were analyzed and identified according to physicochemical properties and spectrum data（MS,hydrogen spectrum and carbon spectrum）. RESULTS：Ten compounds were isolated from the ethanol extract of R. multibracteatus stem,i.e. 5,4′-dihydroxy-8-（3,3-dimethylally）-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone（1）,3-hydroxy-1-（4′-hydroxy-3′-methoxyphenyl）propan-1-one（2）,3β-hydroxysitost-5-en-7-one（3）,Lupeol（4）,Coniferaldehyde（5）,E-p-hydroxy-coumaric acid（6）,Genistein（7）,1-O-p-coumaroylglycerol（8）,Scopoletin（9）,and Kaempferol（10）. CONCLUSIONS：Compound 1-9 are isolated from the plants of R. multibracteatus for the first time,and Compound 2,5,8 are isolated from the plants of Rubus L. for the first time. The study lays the foundation for further development and utilization of R. multibracteatus.