XRCC1基因rs25487位点多态性与肺癌发生的相关性分析

Correlation Analysis of XRCC1 rs25487 Polymorphism with the Occurrence of Lung Cancer

目的:探讨DNA修复基因X线损伤修复交叉互补基因1(XRCC1)rs25487位点多态性与肺癌发生的相关性。方法:选取2015年9月-2016年7月于徐州医科大学附属医院就诊的苏北地区汉族原发性肺癌患者208例,作为肺癌组;选择同期于该院进行体检的健康志愿者214例,作为对照组。采用聚合酶链反应-限制性片段长度多态性法检测各受试者XRCC1基因rs25487位点的基因型,采用Logistic回归模型评价各基因型与肺癌发生的相关性。结果:两组受试者的年龄及性别分布比较,差异均无统计学意义(P>0.05);而肺癌组吸烟者的比例显著高于对照组,差异有统计学意义(P<0.05)。共检出XRCC1基因rs25487位点AA、AG、GG 3种基因型。其中,对照组受试者AA、AG、GG型频率分别为43.5%、41.1%、15.4%,肺癌组患者上述基因型频率分别为28.8%、48.6%、22.6%,两组受试者各基因型频率均符合Hardy-Weinberg平衡(P>0.05),但其基因型分布组间比较差异有统计学意义(P<0.05)。与AA型个体比较,AG型个体发生肺癌的风险增加了2.265倍[比值比(OR)=2.265,95%置信区间(CI)(1.299,3.950),P=0.040;经年龄、性别、吸烟史校正的OR=2.309,95%CI(1.274,4.185),P=0.006],差异有统计学意义;GG型个体发生肺癌的风险增加了1.310倍[OR=1.310,95%CI(0.771,2.228),P=0.318;经校正的OR=1.429,95%CI(0.811,2.518),P=0.217],但差异无统计学意义。结论:XRCC1基因rs25487位点突变杂合是我国苏北地区汉族人群发生肺癌的危险因素,且吸烟可以增加肺癌发生的风险。

OBJECTIVE：To investigate the correlation of XRCC1 rs25487 polymorphism with the occurrence of lung cancer.METHODS：A total of 208 patients with primary lung cancer of Han nationality in Northern Jiangsu selected from the Affiliated Hospital of Xuzhou Medical University during Sept. 2015-Jul. 2016 were included in lung cancer group. A total of 214 healthy volunteers of the hospital underwent physical examination were included in control group. PCR-RFLP was used to detect the genotypes at XRCC1 rs25487 locus,and Logistic regression model was used to evaluate the correlation of genotypes with the occurrence of lung cancer. RESULTS：There was no statistical significance in the distribution of age and gender between 2 groups（P〉0.05）. The proportion of smoker in lung cancer group was significantly higher than control group,with statistical significance（P〈0.05）. AA,AG and GG genotypes were detected at rs25487 locus of XRCC1 gene. The frequency of AA,AG and GG genotype were 43.5%,41.1% and 15.4% in control group and 28.8% ,48.6% and 22.6% in lung cancer group,respectively. The frequencies of genotypes in 2 groups were in line with Hardy-Weinberg equilibrium（P〉0.05）,but there was statistical significance in genotype distribution between 2 groups（P〈0.05）. Compared with AA genotype,the risk of lung cancer in individuals carrying AG genotype increased by 2.265 fold [OR=2.265,95%CI（1.299,3.950）,P=0.040;after corrected with gender,age and smoking history OR=2.309,95%CI（1.274,4.185）,P=0.006],with statistical significance. The risk of lung cancer in individuals carrying GG genotype increased by 1.310 fold [OR=1.310,95% CI（0.771,2.228）,P=0.318;after corrected OR=1.429,95% CI（0.811,2.518）,P=0.217],without statistical significance. CONCLUSIONS：rs25487 locus mutant heterozygosity of XRCC1 gene is risk factor of lung cancer in Han nationality from Northern Jiangsu,and smoking can increase the risk of lung cancer.