响应面法优化马铃薯茎段高效再生体系

Optimization of efficient regeneration system in stem of potato using response surface methodology

为了探讨马铃薯茎段愈伤组织高效再生的最佳激素浓度配比,建立马铃薯组织培养和快速繁殖技术新体系,以马铃薯品种川芋10号茎段为外植体诱导的愈伤组织为材料,通过采用响应面方法(RSM)中的Box-Behnken设计方法,研究TDZ、2,4-D、GA_3浓度对马铃薯茎段愈伤组织芽分化的影响,优化马铃薯愈伤组织芽分化的最佳激素配比。结果表明,从响应面法优化所得模型可知,培养基中的激素浓度变化对马铃薯愈伤组织出芽敏感性TDZ>GA_3>2,4-D;所得模型的决定系数R^2为0.9921,确定了川芋10号茎段愈伤组织芽分化最佳培养基为MS+2.02 mg·L^(-1)TDZ+0.08 mg·L^(-1)2,4-D+2.25 mg·L^(-1)GA_3,在此条件下,实际测得的平均出芽率与预测值基本一致,证实了该模型的有效性。

To establish the optimal hormone concentrations of culture medium for efficient regeneration of potato stem callus tissue,as well as build a new system for tissue culture and rapid propagation technology,stem of Chuanyu No.10 was employed as explants to induce callus tissue,and the Box-Behnken design method of response surface methodology（ RSM） was used to research the effect of TDZ,2,4-D and GA_3 on the differentiation of potato callus. Meanwhile,the hormone concentrations were optimized. The results showed that the sensitivity of the hormone concentrations in culture medium to potato callus budding from the response surface method optimization model is TDZ〉 GA,3 2,4-D. The determination conefficient R^2 of the model was 0. 992 1,the optimal medium for adventitious bud differentiation from stem callus of Chuanyu No. 10 was MS ＋ 2. 02 mg·L^（-1） TDZ ＋ 0. 08 mg·L^（-1）2,4-D ＋ 2. 25 mg·L^（-1） GA_3. In this condition,the budding rate was actual measured,which was consistent with the predicted values. That confirmed the validity of the model.