感染鸭源小鹅瘟病毒QH-L01株樱桃谷雏鸭病理学研究

Pathological study on duck-origin goose Parvovirus strain QH-L01 infection of Cherry Valley duck

探究人工感染条件下,鸭源小鹅瘟病毒QH-L01株对樱桃谷雏鸭各器官组织病理学变化的影响,以期阐明QH-L01的病理发生规律及特征。实验组以每只0.2 m L(10^(6.54)ELD_(50))的剂量肌肉接种30只2日龄樱桃谷肉鸭鸭源小鹅瘟病毒,对照组的鸭接种等量的灭菌生理盐水,分别在1、3、6、9 d观察各组织器官的病理变化。感染后1 d,先出现肺点状出血,部分肺小叶扩张,十二指肠、回肠和盲肠等肠道组织绒毛上皮细胞坏死脱落。感染后3 d,脑、肾、胸腺、法氏囊出现病理变化,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层轻度坏死、脱落。感染后6 d,脾脏淋巴细胞增多,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层中度坏死、脱落。感染后9 d,各组织病变减轻或者恢复正常。整个感染期死亡率为20%。

In the present study,the histopathological changes of various organs of Cherry Valley ducklings were explored under the condition of artificial infection,to clarify the pathogenesis and characteristics of duck-origin goose Parvovirus strain QH-L01.Thirty Cherry Valley ducks in experimental group were intramuscularly inoculated with0.2 m L of 10^（6.54）ELD_（50）duck-origin goose Parvovirus strain,and the ducks in the control group were intramuscularly inoculated with 0.2 m L of sterilized normal saline per duck,to observe the pathological changes of various tissues/organs in each infective stage at 1,3,6,9 d,respectively.On the 1^（st）day after infection,the lungs appeared punctate hemorrhage,partial lobular expansion,and intestinal tissue villous epithelial cells necrosed.On the 3^（rd）day after infection,brain,kidney,thymus,bursa fabricius showed pathological changes,and villus of duodenum and ileum slight necrosed,mucosa layer of cecum appeared slight necrosis.On the 6^（th）day after infection,lymphocytes in spleen increased,moderate necrosis was detected in villus of duodenum,ileum and mucosa layer of cecum.On the9^（th）day after infection,the lesions were reduced or returned to normal.The mortality rate was 20%during the whole infection period.