黄淮白山羊肠道黏膜组织结构及杯状细胞分布特点

Mucous membrane organization structure characteristics and goblet cells distribution observation of Huanghuai white goat's intestinal tract

为探明黄淮白山羊肠道黏膜组织结构和杯状细胞分布特点,取成年健康黄淮白山羊3头,经麻醉后颈静脉放血致死,立即取小肠和大肠各3段,投入到4%多聚甲醛固定液中固定72 h,制作组织切片,经HE和PAS染色后,观察黄淮白山羊小肠和大肠黏膜组织结构和杯状细胞分布特点。结果表明,黄淮白山羊十二指肠和空肠的上皮细胞呈高柱状,固有层分布有较多的淋巴细胞,回肠黏膜的上皮细胞呈低柱状,黏膜下层中含大量的集合淋巴小结,盲肠、结肠和直肠的上皮细胞呈低柱状,大肠腺发达,腺体间弥散着淋巴细胞,直肠的固有层中可见淋巴小结,上皮之间的杯状细胞呈顶浆分泌的形式分泌黏多糖。黄淮白山羊小肠杯状细胞分泌的黏多糖以中性为主,大肠杯状细胞分泌的黏多糖以酸性为主。大肠三段杯状细胞数量极显著(P<0.01)高于小肠杯状细胞,大肠中结肠杯状细胞数量显著(P<0.05)高于盲肠和直肠,小肠中回肠杯状细胞数量显著(P<0.05)高于十二指肠和空肠。因此,黄淮白山羊从十二指肠到回肠,上皮细胞由高柱状逐渐向低柱状变化,杯状细胞主要分布于大肠,在结肠中数量最多。

To observe mucous membrane organization structure characteristics and goblet cells distribution of Huanghuai white goat＇s intestinal tract,three adult healthy Huanghuai white goats were bleed from jugular vein to death,the samples of duodenum,jejunum,ileum,cecum,colon and rectum were taken immediately and immersed into 4%paraformaldehyde fixative to be fixed for 72 h. Tissue slices were thus made,and stained with HE and PAS. The results showed that epithelial cells of the duodenum and jejunum of Huanghuai white goat were highly columnar,and lymphocytes were distributed in lamina propria. The epithelial cells of the ileum mucosa were low columnar,and a large collection of lymph nodes were distributed in submucosa. The epithelial cells of cecum,colon and rectum were also low columnar,large intestinal gland were developed,and there were diffused lymphocytes between glands. Thelymphoid nodule was seen in the lamina propria of rectum. The goblet cells in the epithelium secreted mucopolysaccharide in the form of apical secretion. The small intestinal goblet cells of Huanghuai white goat secreted neutral-mucopolysaccharide,while the large intestinal goblet cells of Huanghuai white goat secreted acid-mucopolysaccharide.The goblet cell＇s number of large intestine was significantly（ P〈0. 01） higher than that of small intestine. The goblet cell number of colon were significantly（ P〈0. 05） higher than those of the cecum and rectum,and the goblet cell number of ileum were significantly（ P〈0. 05） higher than those of the duodenum and jejunum. Therefore,from duodenum to ileum,the epithelial cells gradually changed from high column to low column,the goblet cells were mainly distributed in the large intestine,and the highest number of goblet cells was found in the colon.