过敏性紫癜患儿外周血中SOCS1、SOCS3对CD4^+T细胞分化的影响

Effects of SOCS1 and SOCS3 in peripheral blood on CD4^+T cell differentiation in children with Henoch-Schonlein purpura

目的:研究过敏性紫癜患儿外周血中细胞因子信号转导抑制蛋白1(SOCS1)、SOCS3对CD4^+T细胞分化的影响。方法:选择自贡市妇幼保健院在2014年6月~2018年2月期间收治的过敏性紫癜患儿作为研究的HSP组、同期体检的健康儿童作为研究的对照组。取外周血并测定SOCS1、SOCS3的表达量以及CD4^+T细胞亚群的含量,取血清并测定CD4^+T细胞因子的含量。结果:HSP组患儿外周血中SOCS1、SOCS3的mRNA表达量及SOCS3/SOCS1的比例均显著高于对照组;HSP组患儿外周血中Th1、Treg的含量以及血清中IFN-γ、TGF-β1的含量低于对照组,Th2、Th17的含量以及血清中IL-4、IL-5、IL-17的含量高于对照组且高SOCS3/SOCS1比例的HSP患儿外周血中Th1、Treg的含量以及血清中IFN-γ、TGF-β1的含量低于低SOCS3/SOCS1比例的HSP患儿,外周血中Th2、Th17的含量以及血清中IL-4、IL-5、IL-17的含量高于低SOCS3/SOCS1比例的HSP患儿。结论:过敏性紫癜患儿外周血中SOCS1、SOCS3表达的改变能影响CD4^+T细胞的分化。

Objective：To study the effects of suppressor of cytokine signaling 1（SOCS1）and SOCS3in peripheral blood on CD4＋T cell differentiation in children with Henoch-Schonlein purpura.Methods：Children with Henoch-Schonlein purpura who were treated in Zigong Maternal and Child Health Hospital between June 2014and February 2018were selected as the HSP group of the study,and healthy children who received physical examination during the same period were selected as the control group of the study.Peripheral blood was collected to determine the expression of SOCS1and SOCS3as well as the contents of CD4＋T cell subsets,and serum was collected to determine the contents of CD4＋T cytokines.Results：SOCS1and SOCS3mRNA expression levels as well as SOCS3/SOCS1ratio in peripheral blood of HSP group were significantly higher than those of control group（P〈0.05）;Th1and Treg contents in peripheral blood as well as IFN-γand TGF-β1contents in serum of HSP group were lower than those of control group whereas Th2and Th17contents in peripheral blood as well as IL-4,IL-5and IL-17contents in serum were higher than those of control group（P〈0.05）,and Th1and Treg contents in peripheral blood as well as IFN-γand TGF-β1contents in serum of HSP children with high SOCS3/SOCS1ratio were lower than those of HSP children with low SOCS3/SOCS1ratio whereas Th2and Th17contents in peripheral blood as well as IL-4,IL-5and IL-17contents in serum were higher than those of HSP children with low SOCS3/SOCS1ratio（P〈0.05）.Conclusion：Changes in SOCS1and SOCS3expression in peripheral blood of children with Henoch-Schonlein purpura can affect the differentiation of CD4＋T cells.