肾上腺皮质肿瘤中mTOR信号通路的表达及其与细胞侵袭、血管新生的关系

The expression of mTOR signaling pathway in adrenocortical tumour and its correlation with cell invasion and angiogenesis

目的:研究肾上腺皮质肿瘤中mTOR信号通路的表达及其与细胞侵袭、血管新生的关系。方法:选择在我院手术切除的肾上腺皮质恶性肿瘤组织以及正常肾上腺皮质组织,抽提蛋白后测定mTOR信号分子的表达量,抽提RNA后测定细胞侵袭分子、血管新生分子的mRNA表达量。结果:肾上腺皮质恶性肿瘤组织中p-PI3K、p-AKT、p-mTOR、p-S6K1、p-4E-BP1的蛋白表达量以及MMP2、MMP9、PKCα、VEGF、nm23-H1的mRNA表达量均显著高于正常肾上腺皮质组织,PCAF、E-cadherin、TSP1的mRNA表达量显著低于正常肾上腺皮质组织;Pearson相关性分析显示:肾上腺皮质恶性肿瘤组织中p-PI3K、p-AKT、p-mTOR、p-S6K1、p-4E-BP1的蛋白表达量与PCAF、E-cadherin、TSP1的mRNA表达量呈负相关,与MMP2、MMP9、PKCα、VEGF、nm23-H1的mRNA表达量呈正相关。结论:肾上腺皮质恶性肿瘤中mTOR信号通路的过度激活能够促进细胞侵袭及血管新生。

Objective：To study the expression of mTOR signaling pathway in adrenocortical tumour and its relationship with cell invasion and angiogenesis.Methods：Adrenocortical malignant tumor tissues and normal adrenal cortex tissues surgically removed in the Third People＇s Hospital of Huizhou Guangdong Province between September 2014 and February 2018 were selected,the protein was extracted to determine the expression of mTOR signaling molecules,and the RNA was extracted to determine the mRNA expression of cell invasion molecules and angiogenesis molecules.Results：p-PI3 K,p-AKT,pmTOR,p-S6 K1 and p-4 E-BP1 protein expression as well as MMP2,MMP9,PKCα,VEGF and nm23-H1 mRNA expression in adrenocortical malignant tumor tissue were significantly higher than those in normal adrenal cortex tissue whereas PCAF,Ecadherin and TSP1 mRNA expression were significantly lower than those in normal adrenal cortex tissue;Pearson correlation analysis showed that p-PI3 K,p-AKT,p-mTOR,p-S6 K1 and p-4 E-BP1 protein expression in adrenocortical malignant tumor tissue were negatively correlated with PCAF,E-cadherin and TSP1 mRNA expression,and positively correlated with MMP2,MMP9,PKCα,VEGF and nm23-H1 mRNA expression.Conclusion：The excessive activation of mTOR signaling pathway in adrenocortical malignant tumor can promote cell invasion and angiogenesis.