束花石斛提取物对糖尿病模型大鼠血糖血脂的影响

Effect of Dendrobium chrysanthum Lindl. total extract on the blood glucose and blood lipids in diabetic rats

目的研究束花石斛提取物对高脂饮食联合低剂量链脲佐菌素(STZ)诱导的糖尿病模型大鼠血糖血脂的影响。方法采用腹腔注射STZ(35 mg/kg)加高糖高脂饲料喂养SD大鼠,建立糖尿病大鼠模型。糖尿病组大鼠随机分成4组,模型组(5 m L/kg生理盐水)、阳性对照组(罗格列酮0.42 mg/kg)、束花石斛提取物低剂量组(1.08 g/kg)、高剂量组(2.16 g/kg)。连续给药8周后,比较给药前后大鼠的血糖和体质量。测定给药8周后大鼠血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血清蛋白(GSP)、糖耐量(OGTT)、胰岛素及血糖值,计算胰岛素抵抗指数。结果与模型组相比,高剂量组大鼠的血糖值比给药前降低40.6%,差异有统计学意义;高剂量组大鼠的TG、TC和LDL-C显著降低(P<0.05),HDL-C升高。低剂量组TG和LDL-C显著降低(P<0.05),HDL-C升高。与模型组相比,给药组血糖、GSP、胰岛素抵抗指数降低,胰岛素水平升高,糖耐量改善。结论束花石斛对糖尿病大鼠糖脂代谢具有显著调节改善作用,且呈现一定的剂量依赖性。

Objective To investigate the effect of ethanolic extract from Dendrobium chrysanthum Lindl.（ D.chrysanthum） on the blood glucose and blood lipids in diabetic rats. Methods Type 2 diabetes mellitus rats were induced by intraperitoneal injection of 35 mg/kg streptozotocin（ STZ） and a high-carbohydrate/highfat diet. Diabetic rats were randomly divided into four groups： the model group（ 5 m L/kg physiological saline）,the positive control（ 0.42 mg/kg rosiglitazone）,the low-dose group（ 1.08 g/kg D. chrysanthum）,and the high-dose group（ 2. 16 g/kg D. chrysanthum）. The blood glucose and body weight were recorded.After treatment for 8 weeks, the serum TG, TC, HDL-C, LDL-C, GSP, insulin and OGTT were measured.The insulin resistance（ IR） index was calculated. Results Compared with the model group, the blood glucose in the high-dose group was decreased by 40.6% than before administration.The contents of TC,TG and LDL-C were significantly decreased（ P〈0.05）,and HDL-C was increased in the high-dose group.The contents of TG and LDL-C were significantly lowered（ P〈0.05）,and HDL-C were increased in the low-dose group. Compared with the model group, the blood glucose, GSP and insulin resistance index were significantly lowered in administration groups. Meantime, the insulin level was increased and OGTT was improved. Conclusion D.chrysanthum could significantly improve the glucose and lipid metabolic disorder in T2 DM rats in a dose-dependent manner.