羟基红花黄色素A对脑缺血再灌注损伤大鼠脑组织中PDGF含量及PI3K、Akt蛋白表达的影响

Effect of hydroxysafflor yellow A on PDGF content and PI3K and Akt expression in brain tissue of rats with cerebral ischemia-reperfusion injury

目的探讨羟基红花黄色素A对脑缺血再灌注损伤大鼠脑组织中血小板衍生因子(PDGF)含量及PI3K、Akt蛋白表达的影响。方法 120只SD大鼠随机分为假手术组、模型组、给药1组(羟基红花黄色素A组)、给药2组(羟基红花黄色素A+PDGFβ受体拮抗剂组)、给药3组(PDGFβ受体拮抗剂组),每组各24只。采用线栓法制备脑缺血再灌注损伤大鼠模型,并给予相应药物治疗。比较各组大鼠的神经功能缺失评分、脑梗死面积、脑组织含水量、脑组织与血清中PDGF含量及PI3K、Akt蛋白的表达。结果与假手术组比较,模型组神经功能缺失评分、脑梗死面积及脑组织含水量均显著增高(P<0.05)。与模型组比较,给药1组神经功能缺失评分、脑梗死面积及脑组织含水量均显著降低(P<0.01)。但给药2组和给药3组各项指标与模型组比较差异无统计学意义(P>0.05)。与假手术组比较,模型组脑组织及血清PDGF含量显著增高(P<0.01)。与模型组比较,给药1组脑组织及血清PDGF显著增加(P<0.01)。但给药2组和给药3组脑组织及血清PDGF与模型组比较差异无统计学意义(P>0.05)。与模型组比较,给药1组PI3K、Akt阳性细胞吸光度均显著增加(P<0.01)。但给药2组和给药3组PI3K、Akt阳性细胞吸光度与模型组比较差异无统计学意义(P>0.05)。结论羟基红花黄色素A对缺血性脑损伤的神经保护作用可能通过PDGF介导的PI3K/Akt信号转导通路的激活来发挥作用。

Objective To explore the effect of hydroxyl safflower yellow A on PDGF content and PI3K and Akt expression in brain tissue of rats with cerebral ischemia-reperfusion injury. Methods 120 cases of SD rats were randomly divided into the sham operation group, the model group, administration group 1（ hydroxyl safflower yellow A group）,administration group 2（ hydroxyl safflower yellow A＋PDGF β receptor antagonist group） and administration group 3（ PDGF β receptor antagonist group）. A rat model of cerebral ischemia-reperfusion injury was established by thread occlusion method and the corresponding drug treatment was given. Neurological deficit score,infarct size,brain water content,PDGF level in brain tissue and serum,and PI3K and Akt protein expression in each group were measured. Results Compared with the sham group, the neurological deficit score, cerebral infarction area and brain water content in the model group were significantly increased（ P〈0. 01）. Compared with the model group, the neurological deficit score,cerebral infarction area and brain tissue water content were significantly decreased in group 1（ P〈0.01）. However, there was no significant difference between the administration groups 2 and 3 and the model group（ P〈0.05）. Compared with the sham group,the content of PDGF in the brain tissue and serum was significantly increased in the model group（ P〉0.01）. Compared with model group,the PDGF level in brain tissue and serum was significantly increased in administration group 1（ P〈0.01）. However,there was no significant difference in the PDGF level between the administration groups 2 and 3 and the model group（ P〉0.05）. Compared with the model group,the absorbance of PI3K and Akt positive cells was significantly increased in administration group 1（ P〈0.01）. However,the absorbance of PI3K and Akt positive cells did not show significant difference in the administration groups 2 and 3 compared with the model group（ P〉0.05）. Conclusion Hydroxyl safflower yellow A plays a neuroprotective effect on ischemic brain injury,which may be related to activation of PDGF-mediated PI3K/Akt signal pathway.