两种不同来源间充质干细胞的生物学特性比较

A comparative study on biological characteristics of mesenchymal stem cells from two different sources

背景:间充质干细胞来源广泛,不同组织来源的间充质干细胞在生物学特性上可能存在差异。目的:比较脐带间充质干细胞和壁蜕膜间充质干细胞的生物学特性。方法:分离及体外培养脐带间充质干细胞和壁蜕膜间充质干细胞,绘制生长曲线,计算细胞倍增时间和克隆形成率,检测免疫表型、分化能力及分泌因子水平。结果与结论:(1)两种间充质干细胞均为梭形,漩涡状生长;(2)两种间充质干细胞的生长曲线均为S型;(3)第2代和第5代脐带间充质干细胞的倍增时间均短于同代数的壁蜕膜间充质干细胞,差异均有显著性意义(P<0.05);(4)第2代和第5代脐带间充质干细胞的克隆形成率高于同代数的壁蜕膜间充质干细胞,差异均有显著性意义(P<0.05);(5)两种间充质干细胞的表面标记CD73、CD90以及CD105的阳性率均在95%以上,CD34和CD45的阳性率均在1%以下;(6)两种间充质干细胞均可诱导分化为成骨细胞、成脂细胞;(7)脐带间充质干细胞分泌的表皮生长因子水平显著高于壁蜕膜间充质干细胞(P<0.05);脐带间充质干细胞分泌血管内皮生长因子和干细胞生长因子水平显著低于壁蜕膜间充质干细胞(P<0.05);(8)结果提示,两种间充质干细胞具有相似的功能,但脐带组织可能是一个更有应用前景的干细胞来源。

BACKGROUND： Although mesenchymal stem cells（MSCs） from different sources share many similar characteristics, they also exhibit individual properties. OBJECTIVE： To compare the biological characteristics of MSCs derived from umbilical cord and decidua parietalis. METHODS： Growth curve, cell doubling time, clone formation rate, immune phenotype, differentiation capacity and secreted cytokine levels were analyzed in MSCs derived from umbilical cord and decidua parietalis. RESULTS AND CONCLUSION： MSCs from umbilical cord and the decidua basalis exhibited similar morphology, spiral growth, S-shaped growth curve, immunophenotype, and differentiation potentials to osteogenesis and adipogenesis. For two kinds of MSCs, the positive rates of CD73, CD90 and CD105 were over 95% and the positive rates of CD34 and CD45 were below 1%. The growth rate, cell doubling time and clone formation rate of umbilical cord derived MSCs at passages 2 and 5 were significantly higher than those of decidua parietalis derived MSCs at passages 2 and 5（P〈0.05）. The level of epidermal growth factor secreted from umbilical cord MSCs was significantly higher that that from decidua basalis derived MSCs, while the levels of vascular endothelial growth factor and stem cell growth factor from umbilical cord derived MSCs was significantly lower those from decidua basalis derived MSCs（P〈0.05）. These findings indicate that MSCs from both sources have similar biological properties, but umbilical cord derived MSCs are deemed to have better application prospects.