阿托伐他汀对大鼠脑缺血再灌注后脑组织IL-17、IL-10、TNF-α表达的影响

The effect of atorvastatin on expression of IL-17,IL-10,TNF-α of rats brain tissue with cerebral ischemia reperfusion

目的观察并探讨阿托伐他汀对大鼠脑缺血再灌注后脑组织白介素-17(IL-17)、白介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)表达的影响。方法采用随机数字表法将126只健康成年雄性SD大鼠随机分为A、B、C组,应用线栓法对B组和C组制备脑缺血再灌注模型(右侧大脑中动脉闭塞),A组不给予缺血和再灌注处理,仅将右侧颈总动脉暴露。C组大鼠在手术前2 w开始给予10 mg/kg/d阿托伐他汀混悬液灌胃治疗,A组和B组则同时给予0.9%氯化钠溶液灌胃。对A组术后2 h和B组、C组缺血2 h后再灌注的2 h、6 h、12 h、24 h、48 h、72 h进行取样观察。比较3组大鼠神经功能缺损评分,观察脑组织的病理形态,比较3组大鼠的IL-17、IL-10及TNF-α表达水平的变化。结果 B、C组大鼠在相同再灌注时间点的神经功能缺损评分均高于A组(P<0.05),而C组则低于B组(P<0.05)。B组、C组TNF-α、IL-17、IL-10阳性表达细胞数在缺血2 h再灌注不同时间点均高于A组;C组TNF-α、IL-17阳性表达细胞数则低于B组,IL-10高于B组(P<0.05)。结论 TNF-α、IL-10及IL-17均参与了SD大鼠局部缺血再灌注损伤的发生和进展。阿托伐他汀预防性使用具有神经保护作用,可减轻脑缺血再灌注损伤,其作用机制可能与影响和调节TNF-α、IL-10及IL-17的表达水平有关。

Objective To observe and atorvastatin on ischemia-reperfusion rats afterbrain organization interleukin 17 （IL-17）,interleukin 10 （IL-10） and tumor necrosis factor alpha （TNF-α） expression. Methods Using random number table method 126 healthy adult male SD rats were randomly divided into group A,B,C,line switch method was applied in group B and group C preparation of cerebral ischemia reperfusion model （right） of middle cerebral artery occlusion,don’t give ischemia and reperfusion treatment group A,only to expose the right common carotid artery.Rats in group C were given 10 mg/kg/d atorvastatin suspension gavage 2 weeks before surgery,while those in group A and group B were given 0.9% sodium chloride solution.The 2 h ,6 h,12 h,24 h,48 h and 72 h postoperative reperfusion of group A,group B and group C after 2 h postoperative ischemia were sampled and observed.The neurologic deficit score of 3 groups of rats was compared,and the pathological morphology of brain tissue was observed.The expression levels of IL-17,IL-10 and TNF-α were compared in 3 groups of rats. Results Rats in group B and group C had higher neurologic deficit scores at the same time point of reperfusion than those in group A （ P 〈0.05）,while those in group C were lower than those in group B （ P 〈0.05）.The number of TNF-mediated,IL-17 and IL-10 positive expression cells in group B and group C were higher than group A at different time points of 2 h reperfusion.TNF-α and IL-17 positive expression cells in group C were lower than group B,and IL-10 was higher than group B （ P 〈0.05）. Conclusion TNF-α,IL-10 and IL-17 are all involved in the occurrence and progression of ischemia reperfusion injury in SD rats.Preventive use of atorvastatin has a neuroprotective effect,which can reduce cerebral ischemia reperfusion injury,and its mechanism may be related to the effect and regulation of tnf-length,IL-10 and IL-17 expression levels.