不同质量浓度脂多糖对人肺上皮细胞活性的影响

Effects of Different Concentrations of Lipopolysaccharide on the Activity of Human Lung Epithelial Cells

目的通过不同质量浓度脂多糖(LPS)诱导肺上皮细胞,观察细胞活性的变化,为急性肺损伤(ALI)细胞实验提供基础数据。方法用不同质量浓度LPS(0.050,0.100,0.125,0.250,0.500,1.000,5.000,10.000,50.000,100.000μg/m L)刺激人肺上皮细胞,建立ALI细胞模型,48 h后应用CCK-8试验检测LPS对人肺上皮细胞的活性的影响,并采用RT-PCR法测定细胞间黏附分子-1(ICAM-1)m RNA表达量。结果 10μg/m L LPS可诱导肺上皮细胞活性明显下降,ICAM-1 m RNA表达量显著升高(P<0.05),但细胞坏死不明显。当LPS质量浓度高于50μg/m L时肺上皮细胞活性持续下降,细胞坏死比较明显。结论 LPS质量浓度控制在10~50μg/m L适合ALI细胞模型。

Objective To induce the lung epithelial cells by different concentrations of lipopolysaccharide（ LPS）, and to observe the changes of cell activity,so as to provide basic data for experiment of acute lung injury cell. Methods Different concentrations of LPS（ 0. 050,0. 100,0. 125,0. 250,0. 500,1. 000,5. 000,10. 000,50. 000,100. 000 μg/m L） were used to stimulate human lung epithelial cell,so as to establish acute lung injury cell models. After 48 h,the effect of LPS on cell activity of human lung epithelial cell was detected by CCK-8,and the expression of ICAM-1 m RNA was measured by RT-PCR method. Results 10 μg/m L LPS could induce decrease of human lung epithelial cell activity, while the expression level of ICAM-1 m RNA was obviously increased（ P 0. 05）, but the cell necrosis was not obvious. When the concentration of LPS was higher than 50 μg/m L, the activity of lung epithelial cell continued to decline,and cell necrosis was more obvious. Conclusion LPS concentration at 10-50 μg/m L may suit for acute lung injury cell model.