美洲黑杨与山杨远缘杂交技术的研究

Technology of Intersectional Crossing between Populus deltoides and P. davidiana

用5种不同处理方法对克服辽宁杨×山杨(正交)和山杨×辽宁杨(反交)的杂交障碍进行研究,并用形态学标记法对杂种进行亲子鉴定。研究结果表明:正交杂种子代表现出偏母本型、中间型和偏父本型3种类型,而反交杂种子代表现出偏母本型和偏父本型两种类型,证明杂种子代是其真正的杂种,辽宁杨与山杨远缘杂交取得了成功,用正己烷熏蒸柱头后再用花粉提取液处理柱头是克服其正、反交杂交障碍的方法之一。

The technology of intersectional crossing between P. deltoides and P. davidiana was studied by five different treatments. And the hybrids and their parents were identified using morphological marker.The results showed that hybrids offspring of P. liaoningensis × P. davidiana were inclined to partial parent type,intermediate type and paternal parent type,while hybrids offspring of P. davidiana × P. liaoningensis were inclined to partial parent type and paternal parent type,which meant the hybrid progeny was true hybrids. The effect was better to break incompatible barriers by fumigating stigma with n-hexane followed by brushing stigma with the fluid extracted from pollen.