高田村长寿老人肠道菌群中的抗氧化乳酸菌分离鉴定及评价

Screening and Evaluating of Antioxidant Lactobacillus from Gut Microbacterial of Gaotian Villager

为分离筛选与长寿相关的乳酸菌,了解长寿机制,以高田村长寿老人的粪便样品为研究对象,基于Illumina Mi Seq测序平台对粪便微生物群落的16S r RNA的V4高变区进行测序,并注释序列物种,分析该地区居民肠道中共有的菌属;用MRS选择培养基,从长寿老人粪便中分离筛选该地区共有的乳酸菌,并评价菌株对模拟胃液,肠液的耐受性,同时评价菌株清除自由基能力。结果表明:共有345 OTU为地区各年龄段居民肠道所共有,分离到的1株发酵乳杆菌1014,对模拟胃液,模拟肠液具有较高的耐受能力,并且发酵上清具有较高的自由基清除能力。本文中的实验结果有助于揭示长寿现象,所筛选的菌株也可作为微生物制剂的潜在开发菌株。

To isolate lactic acid bacteria associated with longevity and understand the mechanism about longevity,the gut microbial community of long living elderly residents of Gaotian village was analyzed systematically using 16 S high-throughput sequencing. Sequences were performed by species annotation and analyzed the mutual species of gut microbacterial of villagers. This study use MRS to isolated lactobacillus and evaluate the ability of tolerance in simulated gastric and intestinal fluid as well as the ability of scavenging in free radical. Results showed there were 345 OTUs shared all ages of villages in Gaotian. Lactobacillus fermentum 1014 had a high ability of tolerance in simulated gastric and intestinal fluid,best antioxidant ability of scavenging free radical. Those results might help to reveal the phenomenon of longevity and L. fermentum 1014 might be considered as a potential probiotic lactic acid bacterium used as a microbial agent.