肝癌细胞HepG2与QGY7701摄取^(99)Tc^m-AMD3100与^(18)F-FDG的对比研究

Comparative Study on Uptake of ^(99)Tc^m-AMD3100 and ^(18)F-FDG from HepG2 and QGY7701 in Hepatoma Carcinoma Cell

目的研究2株肝癌细胞对^(99)Tc^m标记的趋化因子受体-4(CXCR4)受体拮抗剂AMD3100(^(99)Tc^m-AMD3100)摄取的动力学特点,并与^(18)F-FDG比较。材料与方法RT-PCR法检测CXCR4m RNA的表达,Transwell小室检测细胞迁移及侵袭能力,体外核素摄取实验研究^(99)Tc^m-AMD3100和^(18)F-FDG的摄取动力学规律。结果Hep G2细胞CXCR4m RNA表达明显高于QGY7701(P<0.05),体外侵袭实验中Hep G2的OD值高于QGY7701(P<0.05)。^(18)F-FDG细胞摄取实验中,Hep G2在30 min、120 min及150 min时摄取率均高于QGY7701(P<0.05)。^(99)Tc^m-AMD3100摄取实验中,Hep G2在60 min摄取率高于QGY7701(P<0.05)。同一株细胞2种示踪剂摄取率比较,120 min和180 min时,Hep G2细胞中^(18)F-FDG摄取率明显高于^(99)Tc^m-AMD3100(P<0.05),而在30 min和120 min时,QGY7701细胞中^(18)F-FDG摄取率明显高于^(99)Tc^m-AMD3100(P<0.05)。结论尽管2株肝癌细胞中^(99)Tc^m-AMD3100摄取率均低于^(18)F-FDG,但^(99)Tc^m-AMD3100摄取率与CXCR4m RNA表达水平、体外侵袭能力变化趋势一致,说明该受体探针能够在体评估肿瘤细胞CXCR4的表达,有助于判别肝癌细胞侵袭力的高低,可作为研究CXCR4高表达肝癌生物学特性的一种新型特异性显像剂。

Purpose To evaluate the dynamic characteristics of ^99Tc^m-AMD3100 from two hepatoma carcinoma cell（HCC）lines and compare with ^18F-FDG.Materials and Methods Detection of CXCR4m RNA by RT-PCR,detection of cell migration and invasion by transwell compartment and study on the dynamic law of uptake of ^99Tc^m-AMD3100 and ^18F-FDG through in-vitro nuclide uptake experiment.Results The expression of CXCR4m RNA in Hep G2 cells was significantly higher than that of QGY7701（P〈0.05）;in in-vitro invasion assay,OD value of Hep G2 was significantly higher than that of QGY7701（P〈0.05）.In ^18F-FDG cell uptake experiment,the uptake rate of Hep G2 was higher than QGY7701（P〈0.05）at 30 min,120 min and 150 min.In ^99Tc^m-AMD3100 cell uptake experiment,the uptake rate of Hep G2 was higher than QGY7701 at 60 min（P〈0.05）.In comparison of the uptake rate of 2 tracers in the same cell,the uptake rate of ^18F-FDG was higher than that of ^99Tc^m-AMD3100 in Hep G2 at 120 min and 180 min（P〈0.05）and the uptake rate of ^18F-FDG was higher than that of ^99Tc^m-AMD3100 in QGY7701 at 30 min and 120 min（P〈0.05）.Conclusion There is the positive correlation between uptake rate of ^99Tc^m-AMD3100 and the expression level of CXCR4m RNA and the variation tendency of invasion in vitro,although the uptake rate of ^99Tc^m-AMD3100 is lower than that of ^18F-FDG in two HCC cells,which shows the receptor probe can evaluate the expression of CXCR4 in tumor cells in vivo,helpful to distinguish the invasiveness of HCC and can be used as the novel specific imaging agent for study on high expression of CXCR4 on biological characteristics of HCC.