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脱氢表雄酮对骨髓间充质干细胞成骨分化相关基因表达的影响

Effects of Dehydroepiandrosterone on Gene Expression of the differentiation of bone marrow mesenchymal stem cells into osteoblasts
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摘要 目的使用qRt-PCR技术,分析脱氢表雄酮对骨髓间充质干细胞成骨分化过程中相关成骨特征性基因表达的变化及其可能的机制。方法提取3~7天SD乳鼠干细胞,分为空白组和脱氢表雄酮组,分别处理4、7、14天后,采用茜素红验证干细胞成骨分化效果,提取各组总RNA行PCR检测相关成骨基因的表达。结果与空白组比较,脱氢表雄酮组在第14天表现出明显的矿化结节,其中成骨特征性基因Alp、Ocn和ColⅠ较对照组明显上调。结论脱氢表雄酮对干细胞成骨分化有明显促进作用,相关骨形成基因显著上调,并可能通过BMP/RUNX2构成的信号通路调节干细胞的成骨分化。 Objective The qRT-PCR technique was used to analyze the changes in the expression of genes related to osteogenic differentiation of BMSCs during osteogenic differentiation of bone marrow mesenchymal stem cells and possible mechanisms. Methods 3~7 days old SD rat neonatal rat stem cells were divided into blank group and dehydroepiandrosterone-treated group.After 4,7 and 14 days of treatment,alizarin red was used to verify the osteogenic differentiation of stem cells.Total RNA was extracted from each group to detection related osteogenic gene expression. Results Compared with the blank group,the DHEA induction group showed obvious mineralized nodules on the 14 th day,among which the osteogenic characteristic genes Alp,Ocn,and ColI were significantly up-regulated in the control group. Conclusion Dehydroepiandrosterone significantly promotes the osteogenic differentiation of stem cells,significantly up-regulates the related bone formation genes,and may regulate the osteogenic differentiation of stem cells through the signaling pathway composed of BMP/RUNX2.
作者 朱永佳 韦庆军 ZHU Yong-jia;WEI Qing-jun(Department of Orthopaedics Trauma and Hand Surgery,the Second People＇s Hospital of Nanning,Nanning,Guangxi,530031;Department of Orthopaedics Trauma and Hand Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning,Ouangxi,530021,China)
机构地区 南宁市第二人民医院创伤骨科手外科 广西医科大学第一附属医院创伤骨科手外科
出处 《蛇志》 2018年第2期169-171,共3页 Journal of Snake
基金 国家自然科学基金(编号:81560371)
关键词 脱氢表雄酮 骨髓间充质 干细胞 成骨细胞分化 DH EA Bone marrow mesenchymal stem cells Osteoblasts Differentiation
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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  • 1Albagha, O.M., Wani, S.E., Visconti, M.R., Alonso, N., Goodman, K., Brandi, M.L., Cundy, T., Chung, P.Y., Dargie, R., Devogelaer, J.E,Falchetti, A., Fraser, W.D., Gennari, L., Gianfrancesco, F., Hooper, M.J., Van Hul, W., lsaia, G., Nicholson, G.C., Nuti, R., Papapoulos, S., Montes, J.del P., Ratajczak, T., Rea, S.L., Rendina, D., Gonzalez- Sarmiento, R., Di Stefano, M., Ward, L.C., Walsh, J.P., Ralston, S.H., Genetic Determinants of Paget's Disease (GDPD) Consortium, 2011. Genome-wide association identifies three new susceptibility loci for Paget's disease of bone. Nat. Genet. 43, 685-689.
  • 2Angers, S., Moon, R.T., 2009. Proximal events in Wnt signal transduction. Nat. Rev. Mol. Cell Biol. 10, 468-477.
  • 3Ardawi, M.S.M., Maimany, A.A., Bahksh, T.M., Nasrat, H.A.N., Milaat, W.A., A1-Raddadi, R.M., 2005. Bone mineral density of the spine and femur in healthy Saudis. Osteoporos. Int. 16, 43-55.
  • 4Arnold, M.A., Kim, Y., Czubryt, M.E, Phan, D., McAnally, J., Qi, X., Shelton, J.M., Richardson, J.A., Bassel-Duby, R., Olson, E.N., 2007. MEF2C transcription factor controls chondrocyte hypertrophy and bone development. Dev. Ceil 12, 377-389.
  • 5Ashford, R.U., Luchetti, M., McCloskey, E.Vi, Gray, R.L., Pande, K.C., Dey, A., Kayan, K., Ralston, S.H., Kanis, J.A., 2001. Studies of bone density, quantitative ultrasound, and vertebral fractures in relation to collagen type I alpha 1 alleles in elderly women. Calcif. Tissue Int. 68, 348-351.
  • 6Aspray, T.J., 2015. Fragility fracture: recent developments in risk assessment. Ther. Adv. Musculoskelet. Dis. 7, 17-25.
  • 7Balemans, W., Ebeling, M., Patel, N., Van Hul, E., Olson, P., Dioszegi, M., Laeza, C., Wuyts, W., Van Den Ende, J., Willems, P., Paes-Alves, A.F., Hill, S., Bueno, M., Ramos, F.J., Tacconi, P., Dikkers, EG., Stratakis, C., Lindpainmer, K., Vickery, B., Foernzler, D., Van Hul, W., 2001. Increased bone density in sclerosteosis is due to the deficiency of a novel secreted protein (SOST). Hum. Mol. Genet. 10, 537-543.
  • 8Banziger, C., Soldini, D., Schutt, C., Zipperlen, P., Hausmann, G., Basler, K., 2006. Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells. Cell 125, 509-522.
  • 9Batra, G.S., Hainey, L., Freemont, A.J., Andrew, G., Saunders, P.T., Hoyland, J.A., Braidman, I.P., 2003. Evidence for cell-specific changes with age in expression of oestrogen receptor (ER) alpha and beta in bone fractures from men and women. J. Pathol. 200, 65-73.
  • 10Berg, J.P., Lehmann, E.H., Stakkestad, J.A., Haug, E., Halse, J., 2000. The Spl binding site polymorphism in the collagen type I alpha 1 (COLIA1) gene is not associated with bone mineral density in healthy children, adolescents, and young adults. Eur. J. Endocrinol. 143, 261-265.

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