烟草β-1,3-葡聚糖酶的特性和结构分析

Characterization and Structure Analysis of Tobacco β-1,3-Glucanase

[目的]对烟草β-1,3-葡聚糖酶的特性和结构进行分析。[方法]以烟草为材料,采用PROTSCALE、Target P 1.1、NPSA-PRABI和SWISS-MODEL工具分别测定烟草葡聚糖酶的疏/亲水性、亚细胞定位、二级结构和三级结构。[结果]NtBGl属于亲水性蛋白,NtBGl-1和NtBGl-2的疏/亲水性较为相似,N端和中部区域的疏水性较强,NtBGl-3以N端的疏水性较强;NtBGl均含有信号肽,且其切割位点分别位于第21、32和29位氨基酸处,NtBGl-1和NtBGl-2定位于液泡,NtBGl-3定位于细胞外;NtBGl的二级结构均以α-螺旋比例最大,依次为36.21%、35.14%、35.28%,三级结构呈现"C"型。[结论]该研究可为有效发挥烟草葡聚糖酶的功能特性奠定基础。

[Objective]To study the characteristics and structure of tobacco β-1,3-glucanase.[Method]Tobacco was used as test material, PROTSCALE, TargetP 1.1, NPSA-PRABI and SWISS-MODEL tools were used to determine the hydrophile/hydrophilicity, subcellular location, secondary structure and tertiary structure of tobacco glucanas. [Result] NtBG1 belonged to the hydrophilic protein, NtBGl-1 and NtBGl-2 had similar sparse/hydrophilic properties, N-terminal and central region had strong hydrophobic, NtBGl-3 only had hydrophobicity at N-terminal. NtBGl contained a signal peptide with cleavage sites at amino acids 21, 32 and 29, NtBGl-1 and NtBGl-2 localized to the vacuole, and NtBGl-3 localized outside the cell. The secondary structure of NtBGl in alpha helix proportion was the largest, followed by 36.21%, 35.14%, 35.28%, The tertiary structure showed “C” type.[Conclusion]The study can lay a foundation for playing the functional properties of tobacco glucanase.