新优绿化植物冬红离体培养与组培快繁技术研究

Technical System of Tissue Culture on Holmskioldia sanguinea Retz.

[目的]建立冬红组培再生体系。[方法]从外植体消毒、外植体出芽诱导、无菌芽继代增殖、壮苗、生根、移栽等方面建立冬红组培再生体系。[结果]用75%乙醇30 s+0.1%HgCl_210 min+0.2%HgCl_25 min进行外植体消毒效果最好;较好的外植体出芽诱导培养基为MS+6-BA 0.2 mg/L+IBA 0.4 mg/L+蔗糖3.0%,诱导率为80%;继代增殖培养基:改良MS+6-BA 0.5 mg/L+KT 0.5 mg/L+NAA0.2 mg/L+蔗糖4.0%的有效苗最多,增殖系数为3.8;壮苗培养基以改良MS+6-BA 0.1 mg/L+NAA 0.2 mg/L+蔗糖3.0%的效果最好;生根培养基1/3MS+NAA 0.5 mg/L+蔗糖1.5%的生根率最高,达85%;移栽基质以泥炭土∶黄泥∶河沙(V∶V∶V=1∶1∶1)的混合基质较好,30 d后移栽成活率高达90%。[结论]该研究基本建立了冬红组培快繁技术体系,为实现冬红组培苗木批量化生产奠定了理论基础。

[Objective]To establish the tissue regeneration system of Holmskioldia sanguinea Retz. .[Method]A technique system of tissue culture of H. sanguinea Retz.was established from the aspect of explant disinfection,sterile bud proliferation,strong seedling, rooting and transplanting.[Result]The best sterilization method was 75% alcohol 30 s＋0.1%HgCl 2 10 min＋0.2%HgCl 2 5 min;explants induction media was MS＋ 6-BA 0.2 mg/L＋ IBA 0.4 mg/L＋ sucrose （3.0%）, induction rate was 80%;subculture multiplication media was modified MS ＋6-BA 0.5 mg/L ＋ KT 0.5 mg/L ＋NAA 0.2 mg/L ＋ sucrose （4.0%）, multiplication coefficients reached 3.8;growth-promoting culture media was modified MS＋6-BA 0.1 mg/L＋NAA 0.2 mg/L ＋sucrose （3.0%）;rooting culture media was 1/3MS＋ NAA 0.5 mg/L ＋sucrose （1.5%）, and the rooting rate was up to 85%;the best transplantation media was peaty soil , yellow soil and fine sand mixture matrix （V ∶V ∶V =1 ∶1 ∶1）, and transplanting survival rate could reach 90% after 30 days .[Conclusion]This study basically established the rapid propagation technology system of H. sanguinea Retz., which laid a theoretical foundation for the realization of the batch production of seedlings of H. sanguinea Retz..