摘要
【目的】探讨低温解除休眠处理后,山桐子种子在萌发过程中内源激素的动态变化及与种子萌发的关系,为明确山桐子种子萌发机制提供依据,也为其繁育与开发应用提供理论基础及技术支撑。【方法】采用酶联免疫法对济源种源山桐子种子进行内源激素含量动态变化的测定。【结果】1)25℃恒温条件下,济源山桐子种子萌发率均低于10%;15℃/25℃变温条件下,没有经历低温处理的山桐子种子发芽率仅为33%~37%,随着5℃低温处理时间的延长,发芽率逐渐升高,60天低温处理的济源山桐子种子发芽率可达98.0%。2)15℃/25℃变温条件下,济源山桐子种子内源激素IAA、GA_3、ZR含量均明显高于25℃恒温,而ABA含量正好相反,(IAA+GA_3+ZR)/ABA或GA_3/ABA比值变大。3)在变温条件下,没有低温处理的山桐子萌发过程中IAA与ABA含量均呈下降趋势,而GA_3与ZR含量均呈先升高再降低的趋势,但经过5℃低温处理的山桐子种子只有ABA含量呈下降趋势,而IAA、GA_3、ZR含量均呈先升高再降低的趋势,差别是峰值出现时间不同。4)不同低温处理时间,(IAA+GA_3+ZR)/ABA与GA_3/ABA的比值在种子萌发过程中均呈先升高再降低的趋势,峰值分别达到3.8±0.2,2.0±0.1,但达到峰值的时间不同,0、20、40和60天的比值达到峰值的时间分别为20、12、12天或8天、8天。5)不同低温处理时间山桐子种子发芽率虽与单一内源激素没有相关性,但与(IAA+GA_3+ZR)/ABA与GA_3/ABA比值均达到显著或极显著相关。【结论】济源山桐子种子具有明显的冬休眠特征,在变温条件下经历不同时间5℃低温解除休眠后的济源山桐子在萌发过程中(IAA+GA_3+ZR)/ABA以及GA_3/ABA比值升高达到峰值的时间越早发芽率越高,当比值达到峰值的时间缩短到8天时,发芽率最大。低温解除休眠的作用是使山桐子种子在萌发过程中及早达到内源激素的平衡态。
【Objective】This study investigated the relationship between the dynamic changes of endogenous hormones and seeds germination after dormancy release to provide a basis for clarifying the mechanism of seed germination of Idesia polycarpa,and to provide a theoretical basis and technical support for the breeding and application of I. polycarpa.【Method】The endogenous hormones contents of I. polycarpa seeds from Jiyuan provenance were determined by enzymelinked immunosorbent assay. 【Result 】1) Under the constant temperature of 25 ℃,the seeds germination rate of I.polycarpa was less than 10%; Under the variable temperature condition of 15 ℃/25 ℃,the germination rate of the seeds without prechilling treatment was 33% 37%; With the extension of the chilling treatment time at 5 ℃,the germination rate increased gradually,and the germination rate reached to 98. 0% in 60 d. 2) Under the variable temperature condition of 15 ℃/25 ℃,IAA,GA3 and ZR contents were significantly higher than that under the constant temperature of 25 ℃.However the ABA content was just the opposite,and the ratio of( IAA + GA3+ ZR)/ABA and GA3/ABA became larger.3) Under the variable temperature condition,IAA and ABA contents showed a decreasing trend,while GA3 and ZRcontents first increased and then decreased during the germination process of seeds without prechilling treatment. But the contents of IAA,GA3 and ZR in seeds prechilled all increased first and then decreased except for ABA,the difference was only the time of peak appearance. 4) With the increase of prechilling time,the( IAA + GA3+ ZR)/ABA and GA3/ABA ratios increased first,respectively reached to 3. 8 ± 0. 2,2. 0 ± 0. 1,and then decreased during the germination process,but time reaching the peak was different among different periods of prechilling,the peak time of prechilling treatment for0,20,40 d and 60 d was at 20,12,12 d and 8 d,respectively. 5) There was no consistent correlation between the seeds germination rate and the single endogenous hormone among the prechilling periods,but the germination rate was significantly correlated with ratios of( IAA + GA3+ ZR)/ABA and GA3/ABA. 【Conclusion 】 The seeds of Idesia polycarpa from Jiyuan provenance have obvious dormancy characteristics. Under the variable temperature condition,experiencing the chilling treatment time at 5 ℃,the earlier the( IAA + GA3+ ZR)/ABA and GA3/ABA ratios in seeds reached to peak,the higher the germination rate was. When the peak time of the ratios was shortened to 8 d,the germination rate was the highest. The effect of prechilling on dormancy release is to reach the equilibrium state of endogenous hormones early in the germination process.
作者
王艳梅
姚兵
刘伟伟
王丽君
颜慧萍
李非
刘震
Wang Yanmei, Yao Bing, Liu Weiwei, Wang Lijun, Yan Huiping, Li Fei, Liu Zhen(College of Forestry, Henan Agricultural University Zhengzhou 45000)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2018年第6期44-52,共9页
Scientia Silvae Sinicae
基金
河南省科技厅农业科技公关项目(182102110070)
林业行业专项(201204806)
关键词
山桐子
休眠解除
萌发
内源激素
Idesia polycarpa
dormancy release
germination
endogenous hormone