二色补血草总黄酮对功能性子宫出血模型大鼠子宫内膜VEGF、MMP-9表达的影响

Effect of Total Flavonoids from Limonium Bicolor on the Expressions of VEGF and MMP-9 in the Endometrium of Rats with Dysfunctional Uterine Bleeding

目的:观察二色补血草总黄酮对功能性子宫出血模型大鼠子宫内膜VEGF、MMP-9表达的影响。方法:大鼠去势并利用雌激素负荷法建立无排卵型功能失调性子宫出血(ADUB)子宫内膜增生症大鼠模型,给予二色补血草总黄酮后,观察大鼠子宫内膜血管内皮生长因子(VEGF)及基质金属蛋白酶-9(MMP-9)表达水平的变化。结果:造模后大鼠VEGF和MMP-9蛋白和m RNA表达水平均显著增加(P<0.01,P<0.05),导致子宫内膜异常增生。给药后,二色补血草总黄酮高剂量组可明显降低大鼠子宫内膜VEGF和MMP-9蛋白和m RNA表达水平(P<0.05);二色补血草总黄酮中剂量组能明显降低大鼠子宫内膜VEGF和MMP-9 m RNA表达水平(P<0.05)和VEGF蛋白水平(P<0.05)。结论:二色补血草总黄酮治疗ADUB的机制可能是通过下调VEGF的转录和翻译水平,引起MMP-9表达水平下降,减轻子宫内膜血管脆性从而达到止血的目的。

Objective： To observe the effect of total flavonoids from Limonium bicolor on the expressions of VEGF and MMP-9 in the endometrium of the rats with dysfunctional uterine bleeding. Methods： The rat model of ADUB hyperplasia endometrii was established by ovariectomy and estrogen load method. The expressions of VEGF and MMP-9 were observed in rats after the intervention of total flavonoids from Limonium bicolor. Results： The protein and m RNA expressions of VEGF and MMP-9 were significantly increased after modeling（ P〈0. 01）,which resluted in abnormal endometrial hyperplasia. The levels of VEGF and MMP-9 in the endometrium of the rats were significantly reduced after the intervention of medium-dose and high-dose of total flavonoids from Limonium bicolor（ P〈0. 05）. Conclusion： The mechansim of total flavonoids from Limonium bicolor treating ADUB may be down-regulating the transcription and translation of VEGF,resulting in the decrease of MMP-9 expression,thus to achieve the purpose of hemostasis by reducing the brittleness of endometrial vessels.