米胚多糖的组成及抗氧化性研究

The Composition and Antioxidant Activity of Rice Germ Polysaccharide

为了研究米胚多糖的单糖组分、氨基酸及抗氧化性,采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱法测定米胚多糖的单糖组分;通过氨基酸自动分析仪检测米胚多糖中的氨基酸。结果表明:米胚多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖组成,其物质的量比为0.90∶4.62∶2.02∶1.52∶35.30。米胚多糖检测出17种氨基酸,总氨基酸含量为2.01%。米胚多糖对DPPH自由基清除率为43.03%,对超氧阴离子自由基的清除率为47.84%,对羟自由基清除率为64.12%;还原力随浓度的增加而增强。米胚多糖可提高小鼠血清及组织中的SOD、T-AOC、GSH-Px活性,降低MDA含量。米胚多糖在体外和体内均具有一定的抗氧化性。

This paper mainly analysed the monosaccharide composition analysis,amino acid analysis and antioxidant activity of rice germ polysaccharides. The monosaccharide components of rice germ polysaccharide were determined by 1-phenyl-3-methyl-5-pyrazolone column pre-column derivatization with high performance liquid chromatography. The amino acids in the rice germ polysaccharide were detected by amino acid automatic analyzer. The results showed that the rice germ polysaccharides consisted of mannose,rhamnose,glucuronic acid,galacturonic acid and glucose with the ratio of0. 90∶ 4. 62∶ 2. 02∶ 1. 52∶ 35. 30. Rice germ polysaccharide detected 17 kinds of amino acids,total amino acid content of2. 01%. The DPPH radical scavenging rate of rice germ polysaccharide was 43. 03%,the scavenging rate of superoxide anion free radical was 47. 84%,the scavenging rate of hydroxyl radical was 64. 12%,and the reducing power increased with increasing concentration. Rice germ polysaccharide can improve the activities of SOD,T-AOC and GSH-Px in the serum and tissues of mice and decrease the content of MDA. Rice germ polysaccharide,in vitro and in vivo,has a certain degree of antioxidant.