辣椒素预防大鼠胃缺血再灌注损伤的作用及机制研究

The Effect and Mechanism of Capsaicin Prevented Acute Gastric Mucosal Injury by Gastric Ischemia-reperfusion

本研究为探讨辣椒素(CAP)对大鼠胃缺血再灌注损伤(GI-R)的作用及机制。成年雄性健康SD大鼠40只,随机分为4组,每组10只,A组(空白对照组),B组(手术组),C组(CAP组),D组(CAP+手术组)。给予A组、B组大鼠灌胃生理盐水10 m L/kg/d,C组、D组大鼠灌胃CAP1 mg/kg/d。4周后B组、D组于GI-R模型建立后24小时处死大鼠,收集胃液测定胃酸总酸度,肉眼计数胃黏膜损伤指数,行胃窦组织病理学观察,检测胃黏膜辣椒素受体(TRPV1)、降钙素基因相关肽(CGRP)的表达,检测胃窦组织丙二醛(MDA)、超氧化物歧化酶(SOD)的水平。结果显示A组、C组胃黏膜未见损伤;与B组相比,D组胃黏膜损伤程度显著减轻(P<0.05),胃酸总酸度显著降低(P<0.05),MDA值显著降低(P<0.05),SOD值显著升高(P<0.05),TRPV1、CGRP表达显著增多(P<0.05)。实验结果表明预先给予CAP 1 mg/kg/d连续灌胃4周具有预防缺血再灌注致大鼠胃黏膜损伤的作用,其机制可能与CAP上调TRPV1、CGRP在胃内的表达、减轻氧自由基损伤有关。

This study was to explore the effect and mechanism of capsaicin on gastric ischemia-reperfusion injury in rats.Specific methods were as follows： 40 SD rats were randomly divided into 4 groups with 10 rats in each group,group A（ control group）,group B（ operation group）,group C（ CAP group） and group D（ CAP ＋ operation group）. Rats in group A and group B were treated with intragastric administration of normal saline 10 ml/kg/d,group C and group D with intragastric administration of CAP1 mg/kg/d. After 4 weeks,the rats in group B and group D were killed 24 hours later after the establishment of GI-R model. Last,astric juice was collected to determine the total acidity of gastric acid,counted the gastric mucosal injury index,observed the gastric mucosa pathological injury,and detected the expression of TRPV1、CGRP、MDA、SOD.Results show that： the gastric mucosa of group A and C had no damage. Compared with group B,the gastric mucosa injury were significantly reduced（ P 〈 0. 05）,total acidity decreased significantly（ P 〈 0. 05）,MDA decreased significantly（ P 〈 0. 05）,SOD increased significantly（ P 〈 0. 05） and the expression of TRPV1、CGRP increased significantly（ P 〈 0. 05） in group D. Experimental results show that： it could prevent that GI-R induced acute gastric mucosal injury in rats by pretreated with CAP 1 mg/kg/d for 4 weeks. The mechanism may be related to the up regulation of TRPV1 and CGRP expression in the stomach and the reduction of oxygen free radical damage by CAP.