生长分化因子15基因的克隆及重组原核表达载体的构建

Cloning of Human Growth Differentiation Factor 15 Gene and Construction of Recombinant Prokaryotic Expression Vector

为克隆人生长分化因子15基因,构建h GDF15基因的重组原核表达载体,为研究该基因的功能奠定实验基础,利用PCR技术克隆其基因序列,将其分别连接至p ET28a和p Cold I载体,构建重组p ET28a-h GDF15和p Cold I-h GDF15原核表达载体,并进行质粒PCR和双酶切验证以及测序验证。结果表明:重组载体构建成功,为进一步研究h GDF15蛋白的作用机制奠定基础。

For gene clone growth differentiation factor 15,constructing h GDF 15 gene recombinant prokaryotic expression vector,which laid the foundation for the study of the function of this gene,cloning thegene sequences using the PCR technology. It＇s connection to p ET28 a and p Cold I carrier respectively,construct recombinant p ET28 a h GDF15 and p Cold I h GDF15 prokaryotic expression vector,and verified plas-mid PCR and double enzyme digestion and sequencing.The results show that the method of recombinant carrier is successful,which lays a foundation for further research on the mechanism of h GDF15 protein.