不同剂量氨磺必利或利培酮对正常大鼠认知功能的影响

Different dose of amisulpride or risperidone's influence on the cognitive function of normal rat

目的:探讨氨磺必利、利培酮对正常大鼠认知功能相关行为、大脑前额叶皮质(PFC)和海马齿状回(DG)区乙酰胆碱酯酶(ACh E)活性的影响。方法:将40只8周龄雄性SD大鼠随机分为5组:利培酮低剂量和高剂量组、氨磺必利低剂量组和高剂量组及溶媒对照组,每组8只。连续腹腔注射给药45 d后,观察大鼠社交行为,检测大鼠对新物体的优先识别指数,测定PFC和DG区ACh E活性。结果:高剂量利培酮组或高剂量氨磺必利组社交接触时间减少(P=0.003,P=0.018);低或高剂量利培酮组(P=0.006,P=0.002)和高剂量氨磺必利组(P=0.001)优先识别指数降低;在PFC区低或高剂量利培酮组(P=0.006,P=0.023)和高剂量氨磺必利组(P=0.025)ACh E活性增高,在DG区低或高剂量利培酮组(P均<0.001)ACh E活性增高。结论:长期腹腔注射利培酮或氨磺必利可能对大鼠认知功能有损害作用。

Objective： To explore the effect of amisulpride and risperidone on cognitive function,related behavior change and activity of acetylcholinesterase（ ACh E） in prefrontal cortex（ PFC） and dentate gyrus（ DG） of normal rats. Method： The 8-weeks old Sprague-Dawley male rats were divided into 5 groups（ 8 rats each group）,each group was intraperitoneally injected drug for 45 days. The social behaviors through open field test case were recorded. The priority recognition index to novel object was calculated. The activity of ACh E in prefrontal cortex and dentate gyrus was measured. Results： The duration of social contact in the high dose risperidone group or the high dose amisulpride group significantly decreased（ P = 0. 003,P = 0. 018）; the priority recognition index of the low or high dose risperidone group（ P = 0. 006,P = 0. 023） and high dose amisulpride group（ P = 0. 001） went down; the activity of Ach E in PFC of the low or high dose risperidone group（ P =0. 006,P = 0. 023） and high dose amisulpride group（ P = 0. 025） increased; the activity of ACh E in DG of the low or high dose risperidone group increased（ P〈0. 001,respectively）. Conclusion： The long-term intraperitoneal injection of risperidone or amisulpride may have damaging effect on cognitive function of rats.