脉络宁对MCAO大鼠的神经保护作用及其抗氧化机制研究

Neuroprotective Effect of Mailuoning on MCAO Rats and Its Antioxidant Mechanism

目的:探讨脉络宁对大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)大鼠的抗氧化作用机制。方法:将18只成年SD大鼠随机分成假手术组(control)、模型组(MCAO)、脉络宁组(mailuoning),每组6只。采用线栓法建立大鼠MCAO模型,缺血2 h再灌注24 h。2%2,3,5-氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染液测定大鼠脑梗死体积,荧光法测定活性氧(reactive oxygen species,ROS)的含量,吸光度法测定脑组织中丙二醛(malondialdehyde,MDA)含量以及还原型谷胱甘肽(glutathione,GSH),Western Blot法测定半胱氨酸天冬氨酸蛋白酶-3(cysteinyl aspartate specific proteinase-3,caspase-3)、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)表达量。结果:脉络宁能显著降低MCAO大鼠神经功能损伤,减少脑梗死体积;能够抑制脑组织中ROS含量以及MDA的生成,显著提高GSH水平;能够抑制Bax、caspase-3的表达,并增加Bcl-2的表达。结论:脉络宁对MCAO大鼠的保护作用可能与其抑制氧化应激及细胞凋亡有关。

Objective：To investigate the antioxidant mechanism of mailuoning on MCAO rats. Methods：18 adult SD rats were randomly divided into control group,MCAO group and mailuoning group,6 rats in each group. A rat middle cerebral artery occlusion（MCAO） model was established by suture method,ischemic 2 h and reperfusion 24 h. 2% 2,3,5-triphenyltetrazolium chloride（TTC） was used to measure the cerebral infarct volume in rat. The content of reactive oxygen species（ROS） were determined by fluorescence spectrometry. The contents of malondialdehyde（MDA） and glutathione（GSH） in brain tissue were determined by absorbance method. The expression of cysteinyl aspartate specific proteinase（caspase-3）,B-cell lymphoma-2 associated X protein（Bax）,B-cell lymphoma-2（Bcl-2） were measured by Western Blot. Results：Mailuoning could significantly decrease the neurological function of MCAO rats and decrease the volume of cerebral infarction. Mailuoning could inhibit the production of ROS and the formation of MDA,meanwhile increase the level of GSH in brain tissue. Mailuoning could also inhibit the expression of caspase-3,Bax,however,increase the expression of Bcl-2. Conclusion：The protective effect of mailuoning on MCAO rats may be related to its inhibition of oxidative stress and apoptosis.