4种胡椒科植物粗提物对灰葡萄孢的抑菌活性

In Vitro Antifungal Activity of Four Pepper Plant Seeds Extracts Against Botrytis cinerea Pers

在离体条件下比较了胡椒科荜茇、假蒟、黑胡椒、白胡椒4种植物乙醇提取物对灰葡萄孢的抑菌活性。结果表明:4种植物乙醇提取物对灰葡萄孢发育各阶段具有较强的抑制作用。白胡椒乙醇提取物对菌丝生长的抑制作用最强,EC_(50)为0.007 9 g·m L^(-1),其次为荜茇、黑胡椒和假蒟;当浓度为0.5 g·m L^(-1)时,荜茇和假蒟的乙醇提取物对灰葡萄孢产孢量抑制作用最强,抑制率分别为92.8%和92.0%;黑胡椒和白胡椒乙醇提取物对灰葡萄孢孢子萌发的抑制作用强于荜茇和假蒟;4种植物粗提物浓度高于0.062 5 g·m L^(-1)时,均能完全抑制灰葡萄孢菌核产生。

In order to investigate the antifungal activity of pepper plant seeds extracts against Botrytis cinerea Pers,the ethanol extract of pepper plant seeds was studied against all growth stages of Botrytis cinerea Pers in vitro.The results indicated that 4 pepper plant seeds had strong antifungal activity agaist Botrytis cinerea Pers. The white pepper extract showed more inhibition on mycelium growth of Botrytis cinerea Pers than that of Piper longum L.,black pepper and Piper sarmentosum Roxb. with EC50 at 0.007 9 g·mL^-1.The inhibition ratios of extracts of Piper longum L. and Piper sarmentosum Roxb. on spore product were 92.8% and 92.0% at 0.5 g·mL^-1.The antifungal activity of black pepper and white pepper extract on spore germination were higher than that of Piper longum L. and Piper sarmentosum Roxb.Sclerotium product was inhibited,when the extract concentration of 4 pepper plant seeds was higher than 0.062 5 g·mL^-1.