复方苦参注射液对肝癌细胞增殖及自噬的影响

Effects of Compound Kushen Injection on proliferation and autophagy of hepatocellular cancer SMMC-7721 cells

目的研究复方苦参注射液抑制人肝癌细胞SMMC-7721增殖及诱导其自噬的作用,为复方苦参注射液用于肝癌治疗提供依据。方法复方苦参注射液(终浓度为0、0.5、1、2、4 mg/m L)处理人肝癌细胞SMMC-7721 48 h,采用MTT法检测细胞增殖抑制率;用RT-PCR及Western-blot方法检测LC3及SQSTM1的转录水平和蛋白表达水平。结果复方苦参注射液可显著抑制人肝癌细胞SMMC-7721的增殖,作用呈浓度依赖性;RT-PCR方法检测到LC3及SQSTM1的转录水平均上调;Western-blot法检测到LC3蛋白表达上调,SQSTM1蛋白表达下降。结论复方苦参注射液可显著抑制人肝癌细胞SMMC-7721并诱导细胞发生自噬现象。

Objective To study the inhibitory effect of Compound Kushen Injection on the proliferation and induction of autophagy of human liver cancer cell SMMC-7721, and provide basis for the treatment of liver cancer. Methods The human liver cancer cell line SMMC-7721 was treated with Compound Kushen Injection（final concentrations 0, 0.5, 1, 2 and 4 mg/m L）, and the cell proliferation inhibition rate was detected by MTT assay. The transcriptional level and protein expression of LC3 and SQSTM1 were detected by RT-PCR and Western-blot. Results Compound Kushen Injection could significantly inhibit the proliferation of human liver cancer cell line SMMC-7721 in a concentration-dependent manner. The transcriptional levels of LC3 and SQSTM1 were both up-regulated by RT-PCR. The protein expression of LC3 was up-regulated and SQSTM1 protein expression was down-regulated by Western-blot. Conclusion Compound Kushen Injection can significantly inhibit proliferation of human liver cancer cell SMMC-7721 and induce autophagy.