球周注射尼莫地平对兔慢性高眼压致视网膜神经节细胞凋亡的干预作用

Intervention effect of Nimodipine injected around eyeball of rabbit on retinal ganglion cell apoptosis caused by chronic intraocular hypertension

目的观察眼球周注射尼莫地平对慢性高眼压兔网膜视神经节细胞凋亡的干预作用。方法选用健康新西兰白兔30只,随机分为A、B、C、D、E 5组,每组6只。A组为正常组,其余各组均行复方卡波姆复制慢性高眼压模型,B组注射生理盐水,C、D、E组分别球周注射低(0.125 mg)、中(0.250 mg)、高(0.500 mg)剂量尼莫地平(NMD)注射液。第2周处死动物取视网膜组织行免疫组化、TUNEL法检测及HE形态学观察,比较各组间的差异。结果 B淋巴细胞瘤2基因(Bcl-2)在B、C组呈低表达,在D、E两组呈高表达,D、E两组均高于B、C两组,差异有统计学意义(P<0.05)。B淋巴细胞瘤2基因相关蛋白X(Bax)在B、C组呈高表达,随NMD剂量增高阳性细胞表达量降低,D、E组呈低表达,差异有统计学意义(P<0.05)。TUNEL检测,在B、C组呈高表达,在D、E组呈低表达,差异有统计学意义(P<0.05)。结论尼莫地平注射液球周注射对兔慢性高眼压视网膜神经节细胞凋亡有积极的保护作用。

Objective To investigate the intervention effect of Nimodipine injected around eyeball of rabbit on retinal ganglion cell apoptosis caused by chronic intraocular hypertension. Methods Thirty healthy New-Zealand albino rabbits were randomly assigned to 5 groups. Group A is blank control group and the rest of the groups（B, C, D and E） were chronic intraocular hypertension（CIH） ones which were induced by compound Carbomer. Group B were injected by Saline Injection（SI）. Group C, D and E were injected by Nimodipine with low（0.125 mg）, medium（0.250 mg） and high（0.500 mg） dose, respectively. The models were executed at the 2 nd week. The retinal tissues in the groups were detected by immunochemistry, TUNEL and HE to identify the differences. Result The expression level of Bcl-2 in group D and E were significantly higher than that in group B and C（P 〈 0.05）. The expression of Bax in group B and C had a relatively high expression,while the expression of positive cells was decreased with the increase of NMD dose. The expression levels of Bax in group D and E were lower than those in group B and C（P 〈 0.05）. With TUNEL method, the apoptosis expression in group D and E were significantly lower than that in group B and C（P 〈 0.05）. Conclusion Nimodipine injected around eyeball can protect the retinal ganglion cells caused by chronic intraocular hypertension.