仿贻贝磷脂聚合物的合成及其仿生涂层的构建

Construction of bionic coating and synthesis of imitation mussel of phospholipid polymers

为改善纯钛表面的抗凝血性能,合成了一系列磷酸胆碱仿生聚合物,并在其表面构建了稳定的仿生涂层,通过一系列实验、表征和评价优化出更有利于钛材表面改性的磷酸胆碱聚合物。首先采用简单的自由基聚合法和羧基与氨基的反应,分两步合成了5种不同比例的2-甲基丙烯酰氧乙基磷酰胆碱(MPC)、甲基丙烯酸(MA)和多巴胺(DA)的无规共聚(PMMDA3-Q、PMMDA3-T、PMMDA5-Q、PMMDA5-T、PMMDA7),并通过FTIR、1 H NMR、GPC以及UV-Vis等完成了定性、定量表征和分析。通过自组装的方式,将5种不同聚合物分别组装到钛表面,并采用QCM-D、AFM及血小板粘附实验考察了5种聚合物在钛表面的组装量、表面粗糙度以及改性后的钛表面抗血小板粘附及抑制血小板激活的能力。结果表明,聚合物PMMDA7更适合作为钛材表面改性聚合物,为构建磷酸胆碱仿生多功能涂层提供了实验基础和方法。

To improving the blood compatibility of pure titanium surface, a series of biomimetic polymers containing phosphorylcholine were synthesized and stable bionic coating on the surface of pure titanium was built. The better phosphorylcholine polymer which was more favorable for surface modification of titanium was optimized through a series of experiments, characterization and evaluation. Firstly, the simple free radical polymerization and the reaction of carboxyl and amino were used to synthesize 5 kinds of random copolymers（hereinafter referred to as PMMDA3-Q, PMMDA3-T, PMMDA5-Q, PMMDA5-T, PMMDA7） with different proportions of 2-methacryloyloxyethyl phosphorylcholine（MPC）, methacrylic acid （MA） and dopamine （DA） in two steps. The qualitative and quantitative characterization and analysis were characterized by means of FT-IR, 1H NMR, GPC and UV-Vis. The polymers were assembled on titanium surface respectively by self-assembly and the quantity, surface roughness and the ability of inhibit platelet adhesion and activation of the five kinds of polymer on the surface of the titanium were inspected by means of QCM-D and AFM. Results showed that the polymer PMMDA7 is more suitable as the titanium surface modification polymer, and the experimental basis and methods to build the phosphorylcholine bionic multi-functional coating were provided.