急性白血病β-catenin与LEF1基因表达的相关性

Expressions of β-catenin and LEF1 in acute leukemia and their correlation

目的探讨β-catenin、淋巴细胞增强因子1(LEF1)在急性白血病(AL)中的表达及相关性。方法实时定量PCR方法检测62例初诊AML患者、23例初诊ALL患者、20例对照及K562、HL-60、THP-1、Raji细胞株β-catenin和LEF1 m RNA表达。结果 AML组β-catenin m RNA表达显著高于对照组,LEF1m RNA表达显著低于对照组(P<0.05)。ALL组β-catenin、LEF1 m RNA表达均高于对照组(P<0.05)。AML、ALL组β-catenin与LEF1 m RNA表达均呈相关性(P<0.05)。在三种AML细胞株中,THP-1细胞β-catenin m RNA表达最高,LEF1 m RNA表达最低,表达方向基本与AML患者一致。结论 AL患者有Wnt/β-catenin路通的异常激活,β-catenin/LEF1转录复合体可能在ALL发病中发挥作用。

Objective To investigate the expressions of β-catenin and lymphoid enhancer factor 1（LEF1）mRNA in acute leukemia（AL）and their correlation. Methods Real-time PCR was used to examine the expres-sions of β-catenin and LEF1 mRNA in 62 de novo acute myeloid leukemia（AML）patients,23 de novo acutelymphoblastic leukemia（ALL）patients,20 controls and 4 kinds of cell lines：K562,HL-60,THP-1 and Raji.Results The expression level of β-catenin in AML group was significantly higher compared with the controlgroup,but LEF1 mRNA expression in AML group was significantly lower than that in the control group（P〈0.05）. The expression levels of β-catenin and LEF1 mRNA were all significantly higher in ALL group comparedwith the control group（P〈0.05）. There were significantly positive correlations between β-catenin and LEF1 mRNA expressions both in AML and in ALL groups（P〈0.05）. Among the three AML cell lines,THP-1 cell hadthe highest β-catenin mRNA expression and the lowest LEF1 mRNA expression,and the expression tendencies ofthese two genes in THP-1 cell line were in accordance with those in AML patients. Conclusions Activation ofWnt/β-catenin pathway happends in AL patients and β-catenin/LEF1 may play a role in leukemogenensis of ALL.