用于胆红素吸附的β-环糊精改性PVDF血浆分离膜的制备

Preparation of PVDF plasma separation adsorption membrane by modifiedβ-cyclodextrin for bilirubin removal

由于传统滤过吸附在两个组件中实现,流程长,感染风险大,因此,制备了一种一步滤过吸附用膜,使滤过吸附在一个组件中完成.本文用PVDF中空血浆分离膜作为基体材料,单取代改性β-环糊精(β-CD)作为胆红素的吸附配基,仅在膜透过侧接枝β-CD,制备了一种新型PVDF血浆分离吸附膜.实验中采用红外光谱、核磁共振波谱等测试手段对改性β-CD和膜的化学成分进行表征分析;用扫描电镜、原子力显微镜等方法验证了改性膜的表面结构及断面结构,泡点测试和通量测试表征改性膜的分离性能;最后通过模拟血浆测试了改性膜对BSA结合胆红素的吸附能力.结果表明,β-CD成功接枝到PVDF血浆分离膜的透过侧;改性膜相比血浆分离膜膜孔大小基本不变,最大孔径为0.480μm,满足细胞不能透过,血浆和胆红素能透过的要求;一步滤过吸附方式的吸附率达到41.2%,与直接吸附(43.5%)的效果基本一致.

Due to the traditional filtration adsorption is achieved in two components with long process and high risk of infection,therefore,a one-step filtration adsorption membrane was prepared,allowing filtration and adsorption to be completed in one module.PVDF hollow plasma membrane was used as the matrix material.Monosubstitutedβ-CD was used as adsorption ligand for bilirubin.A new type of PVDF plasma separation adsorption membrane was prepared for one-step filtration adsorption.The chemical composition of the modifiedβ-cyclodextrin and the membrane was characterized by infrared spectroscopy and nuclear magnetic resonance spectroscopy.Scanning electron microscopy and atomic force microscopy were used to characterize the surface and cross-sectional morphologies and the bubble point test and flux test of the membrane were used to investigate separation performance of the membrane.Finally,the adsorption ability of modified membrane on BSA-bound bilirubin was tested by mimic plasma.The results showed thatβ-CD was successfully grafted on the permeation side of PVDF plasma membrane.The size of modified membrane,with the maximum pore size of 0.480μm,was almost unchanged compared with that of plasma separation membrane.Therefore the blood cells won＇t permeate through modified membrane and plasma and small molecule toxins can permeate.Adsorption rate of 41.2%in one-step filtration adsorption was basically the same as that in direct adsorption（43.5%）.