10%吡丙醚乳油的高效液相色谱分析

Analysis of Pyriproxyfen 10 % EC by HPLC

采用反相高效液相色谱外标法,以乙腈、水作为流动相,用C18不锈钢柱和紫外检测器(230 nm)测定10%吡丙醚乳油的含量,优化了色谱分析条件:检测波长为230 nm,流动相为乙腈—水混合液(V_(乙腈)∶V_水=50∶50),流速为1.0 mL/min。同时,验证了该方法的精密度和准确度。结果表明,方法的标准偏差为0.011 4,变异系数为0.11%,平均回收率为99.63%,线性相关系数为0.999 6。该方法分离完全、峰形对称、线性范围宽,对10%吡丙醚乳油含量的分析具有实际指导意义。

The content of 10 % pyriproxyfen emulsifiable concentrate is determined by reversed-phase high performance liquid chromatography（HPLC） with acetonitrile and water as the mobile phase, C18 stainless steel column and UV detector（230 nm） used to optimize the chromatographic conditions： the detection wavelength is 230 nm; the mobile phase is acetonitrile-water mixture（Vacetonitrile： Vwater = 50：50）; the flow rate is 1.0 mL/min. Meanwhile, the precision and accuracy of the method are verified. The standard deviation of the method is 0.011 4; the coefficient of variation is 0.11 %; the average recovery is 99.63 % and the linear correlation coefficient is 0.999 6. The method has complete separation, symmetrical peak shape and wide linear range. It has practical significance for the analysis of 10 % pyriproxyfen EC content.