Quinadoline C——海南海桑内生真菌的一种新生物碱

Quinadoline C,a new alkaloid from mangrove Sonneratia hainanensis-associated Aspergillus sp.

红树植物内生真菌是寻找结构新颖天然产物新药的重要来源.采用正相、反相硅胶柱色谱和Sephadex LH20凝胶柱色谱法,从海南特有红树植物海南海桑内生曲霉菌菌株Aspergillus sp.HS02的麦芽培养基发酵液乙酸乙酯提取物中,分离得到了化合物1,该化合物为发酵液主要成分.利用高分辨电喷雾质谱、一维核磁共振波谱、异核单量子相关谱、异核多键相关谱和氢-氢质子同核相关谱对化合物1结构进行了鉴定,确定为一个新的吲哚生物碱,命名为quinadoline C.利用滤纸片琼脂扩散法评价了该化合物抗农业病原真菌活性.

Mangrove-derived endophytic fungi are important and promising resources for the novel natural products discovery and have attracted much attention of natural product chemists. A strain of iun^us Aspergillus sp. HS02 was isolated from the leaf of the mangrove plant Sonneratia hainanensis endemic in Hainan Island. Fermentation of this fungus was conducted in liquid malt extract medium （ MEA）. From the ethyl acetate extract of the fermentation broth, one major component 1 was isolated and purified by normal phase, reversed-phase silica gel and sephadex LH20 column chromatography. The structure of compound 1 was identified as a new indole alkaloid by high resolution electrospray ionization mass spectroscopy, one-dimensional nuclear magnetic resonance spectroscopy, heteronuclear single quantum correlation, heteronuclear multiple bond correlation and 1H-1 H correlated spectroscopy. It was named as quinadoline C. The anti-fungi activity of quinado line C against two agricultural pathogenic fungi was evaluated in vitro by filter paper disc diffusion method.