自发性犬骨肉瘤动物模型的免疫组织化学检测

Immunohistochemical detection in dog models of spontaneous osteosarcoma

背景:由于骨肉瘤基因的复杂性,其分子特征至今还没有被完全理解。对于人类骨肉瘤,高表达碱性磷酸酶、转录因子2(Runx2)和骨形态发生蛋白4,部分微表达肌间线蛋白、S100和神经特异性烯醇化酶(NSE),而犬骨肉瘤肿瘤标记物是否与人类相似,尚不确定。目的:对作为人类疾病模型的犬类骨肉瘤进行验证,评估对于人类骨肉瘤来说重要的标记物的表达情况。方法:取59例来自于被证实有原发性骨肉瘤的犬类组织切片,进行免疫组织化学标记,检测碱性磷酸酶、肌间线蛋白、S100、神经特异性烯醇化酶、转录因子2和骨形态发生蛋白4的表达。结果与结论:(1)碱性磷酸酶、转录因子2和骨形态发生蛋白4在所有肿瘤中高度表达,而肌间线蛋白、S100和神经特异性烯醇化酶表达情况多变;(2)研究发现与在人类骨肉瘤中的发现类似,表明犬科动物可用作研究人类疾病的模型。

BACKGROUND： Molecular features of osteosarcoma are not fully understood because of the complexity of osteosarcoma gene. In human osteosarcoma, there are highly expressed alkaline phosphatase, runt-related transcription factor 2（Runx2） and bone morphogenetic protein 4（BMP4）, as well as slightly expressed desmin, S100 and neuro-specific enolase（NSE）. However, whether markers of osteosarcoma are similar to those of human osteosarcoma remains unclear.OBJECTIVE： To verify the canine osteosarcoma experiments simulating human disease model and to assess the expression of important markers for human osteosarcoma. METHODS： Fifty-nine tissue sections of the canine osteosarcoma were used for immunohistochemical staining. The expression levels of alkaline phosphatase, desmin, S100, NSE, Runx2 and BMP4 were detected. RESULTS AND CONCLUSION： Alkaline phosphatase, Runx2 and BMP4 were highly expressed in all tumors, while the expression of desmin, S100 and NSE was variable. The findings are similar to those found in human osteosarcoma, suggesting that canine animals can be used as the models for studying human diseases.