小干扰RNA抑制树突细胞瘤苗白细胞介素-10受体对食管癌细胞免疫效应的研究进展

Research progress of small interfering RNA suppressing the effect of dendritic cell vaccine IL-10 receptor on esophageal cancer cells

食管癌是我国最常见的致死性恶性肿瘤之一,传统手术、放射性治疗及化疗效果难以令人满意。树突细胞(dendritic cell,DC)瘤苗是目前最具前景治疗方式之一,但传统瘤苗效果欠佳,原因可能与其分化抑制有关。其中,白细胞介素-10(IL-10)是免疫抑制作用比较强的一种抑制因子,几乎所有DC细胞和单核巨噬细胞都是IL-10抑制性作用的靶细胞。通过小干扰RNA技术抑制树突细胞IL-10受体的表达,培养IL-10受体缺失的树突细胞,制备负载食管癌细胞抗原的树突细胞,从而制备负载食管癌细胞的DC高效瘤苗,提高DC瘤苗的肿瘤治疗作用,为食管癌的肿瘤免疫治疗和肿瘤基因治疗提供新思路。

Esophageal cancer is one of the most common fatal malignant tumors in our country. Theeffect of traditional surgery, radiotherapy and chemotherapy is still unsatisfactory. DC tumor vaccine is currentlyone of the most promising treatment, however, the traditional tumor vaccine effect is poor, and the reason maybe related to its differentiation inhibition. Among them, IL-10 is one of the most potent inhibitors of immunosup-pression, and almost all of DCs and monocytes are targets of IL-10 inhibitory activity. Through inhibition of IL-10 receptor expression in dendritic cells by siRNA technology, dendritic cells lacking IL-10 receptor are cul-tured. Dendritic cells loaded with esophageal cancer cell antigen are prepared, thereby to prepare a dendritichigh efficiency tumor vaccine loaded with esophageal cancer cells and improve the therapeutic effect of the DCvaccine on tumor as well as to provide a new idea for combining tumor immunotherapy with tumor gene therapyin esophageal cancer.