利用生理生化指标评价花生种质耐低温性（英文）

Evaluation of Groundnut(Arachis hypogaea L.) Germplasm for Chilling Tolerance Utilizing Physiological and Biochemical Parameters

本研究的目的是评估低温条件下种子发芽率和幼苗期生理生化指标和植株恢复情况,以了解参试花生基因型对低温的反应。在中国2个省份(吉林和山东)随机抽取69个花生基因型给予2℃预处理后测定发芽率。根据种子发芽数据选取18个基因型用于苗期低温胁迫实验。播种前,用近红外反射光谱(NIRS)分析其种子蔗糖、总可溶性糖(TSS)、维生素E、芥酸、蛋白质、棕榈酸、油分、亚油酸和油酸含量。通过比较低温处理的幼苗和其对照,发现处理后所有基因型的脯氨酸、丙二醛(MDA)、过氧化物酶(POD)含量均增加,而叶绿素含量均减少。种子品质指标与幼苗生理生化胁迫指标之间无显著正相关。据观察,所有基因型幼苗在遭遇低温胁迫处理后都发生不同响应。本研究表明,基因型16C-4、16C-26、16LT-5、16LT-8、ZH1071、ZH1084、ZH1061、ZH1081和ZH1086耐低温胁迫。研究结果提示,花生对低温胁迫的反应随着植物生长阶段的不同而有所区别。

The aim of this study was to evaluate the germination percentage, biochemical and physiological parameters of seedlings and plant recovery in order to understand the response of selected groundnut genotypes to low temperature. The germination percentage was measured at 2℃ in 69 groundnut genotypes randomly selected from 2 provinces （Jilin and Shandong） in China. The seed germination data collected was used to select 18 genotypes used for chill stress experiment at seedling stage. Prior sowing, the seeds of 18 genotypes were analyzed for sucrose, total soluble sugars （TSS）, vitamin E, erucic acid, protein, palmitic acid, oil, linoleic acid and oleic acid using near infrared reflectance spectroscopy （NIRS）. By contrast between treated seedlings and their control, the increase in proline, malondialdehyde （MDA）, peroxidase （POD） and a decrease in chlorophyll were recorded for all genotypes. There was no significant positive correlation between the seeds quality parameters and seedlings biochemical and physiological stress indicators. The differential biochemical and physiological response of seedlings after chill stress treatment was observed for all genotypes. The current study suggest that, genotypes 16C-4, 16C-26, 16LT-5, 16LT-8, ZH1071, ZH1084, ZH1061, ZH1081 and ZH1086 have tolerance to chill stress. These results furthersuggest that, groundnut response to chill stress differs with the plant growth stage.