花生栽培种γ-TMT基因的克隆和序列分析

Cloning and Sequence Analysis ofγ-Tocopherol Methyltransferase(γ-TMT) Genes from the Cultivated Peanut

生育酚甲基转移酶(γ-TMT)是生育酚生物合成过程中的关键酶,对生育酚组成及活性起着至关重要的作用。本研究从花生栽培种A、B两个基因组中克隆到2个γ-TMT基因,分别命名为Ahγ-TMT1和Ahγ-TMT2。Ahγ-TMT1编码区全长为3111bp,Ahγ-TMT2编码区全长为3124bp,都含有6个外显子和5个内含子,开放阅读框(ORF)长均为1059bp,编码352个氨基酸。利用相关软件或在线工具进行了蛋白质功能预测和系统发育分析。本研究为花生高维生素E分子育种提供了便利。

Tocopherol methyltransferase （ γ-TMT ） is a key enzyme in tocopherol biosynthesis, playing a key role in tocopherol composition and activity. In this study, 2 γ-TMT genes from the A- and B- genomes of the cultivated peanut were identified, which was named as Ahγ-TMT1 and Ahγ-TMT2 . Bioinformatics analysis indicated that the complete coding sequence of Ahγ-TMT1 and Ahγ-TMT2 was 3111bp and 3124bp, respectively, both genes containing 6 exons and 5 introns, and the open reading frame （ORF） length was 1059bp, encoding 352 amino acids. Protein function prediction and phylogenetic analysis were conducted. The study may facilitate peanut molecular breeding for high vitamin E content.