摘要
为研究鞘脂质和鞘脂质代谢前体长链基团(long-chain bases,LCBs)对胁迫应答的调控机制,对编码水稻丝氨酸棕榈酰转移酶(serine palmitoyltransferase,SPT)的LCB基因进行克隆。通过反转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)扩增到3条LCB1和3条LCB2基因全长,蛋白质大小为481~497个氨基酸。生物信息学分析表明,LCB蛋白序列在细菌到人等多个物种中具有高度保守性。在褐飞虱(brown planthopper,BPH)的侵害作用下,水稻Os LCB2a1基因表达量上升。与其他水稻品种相比,抗性品种"Mudgo"和"IR64"中的基因表达量更高。基因表达量与褐飞虱危害指数呈负相关。在不同水稻品种的不同组织中,"IR64"叶片中的基因表达量显著升高,而"态平籼"叶鞘中的基因表达量显著升高。Os LCB2a1基因在抗褐飞虱水稻品种的最高分蘖期呈高表达,表明该基因可能在水稻营养期发挥作用。
To understand potential mechanisms involved in sphingolipids and their metabolic precursors,longchain bases(LCBs)-mediated stress response,we cloned rice LCB genes that encode serine palmitoyltransferase(SPT),which is the key enzyme of sphingolipid de novo biosynthesis.The full-length c DNAs of three LCB1 and three LCB2 genes were amplified by reverse transcription-polymerase chain reaction(RT-PCR).The lengths of the deduced LCB proteins ranged from 481 to 497 amino acid residues.Bioinformatics and comparative studies revealed that the polypeptide proteins had high homology in a large number of species from bacteria to humans.In rice,we found that Os LCB2 a1 expression was up-regulated when infested by brown planthopper(BPH).Compared with other varieties,gene expression levels were higher in BPH-resistant varieties such as Mudgo and IR64.Gene expression was negatively correlated with BPH-resistant scores.Among different plant parts of rice varieties,gene expression was found to be significantly increased in the leaf blade of IR64 and the leaf sheath of Taiping.The m RNA level of Os LCB2 a1 was found to be high at the maximum tillering stage of BPH-resistant varieties,suggesting that this gene may play a role in the vegetative stages of rice.
作者
BEGUM Mahfuj Ara
史肖肖
白月亮
蒋艳冬
周文武
毛存贵
祝增荣
BEGUM Mahfuj Ara;SHI Xiaoxiao;BAI Yueliang;JIANG YANDong;ZHOU Wenwu;MAO Cungui;ZHU Zengrong(Institute oflnsect Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;Department of Medicine and Stony Brook Cancer Center,The State University of New York at Stony Brook,New York 11794,USA)
出处
《浙江大学学报(农业与生命科学版)》
CAS
CSCD
北大核心
2018年第3期365-372,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
supported by the National Natural Science Foundation of China(31572001)
Industry Project of the Ministry of Agriculture of China(201403030)
the National Key Basic Research and Development Project(973 Program)(2010CB126200)
关键词
鞘脂质代谢
丝氨酸棕榈酰转移酶
防御
基因表达模式
sphingolipid metabolism
serine palmitoyltransferase
defense
gene expression pattern