3种杀虫剂亚致死浓度对斑翅果蝇和黑腹果蝇体内解毒酶的影响

Effect of sublethal concentrations of three pesticides on the activity of detoxifying enzymes in Drosophila melanogaster and Drosophila suzukii

为探讨甲维盐、多杀菌素和高效氯氟氰菊酯亚致死剂量对斑翅果蝇和黑腹果蝇解毒酶的影响,本研究在3种杀虫剂亚致死剂量胁迫下,测定了两种果蝇体内乙酰胆碱酯酶(AchE)、谷胱甘肽转移酶(GSTs)、羧酸酯酶(CarE)和多功能氧化酶(MFO)的活性。结果表明,甲维盐可诱导斑翅果蝇体内(AchE)和(GSTs)的活性,而对黑腹果蝇则具有抑制作用;在乙基多杀菌素和高效氯氟氰菊酯亚致死剂量的处理下,两种果蝇体内CarE的活性升高,而黒腹果蝇体内的GSTs活性在剂量为LC20时会显著下降;另外,3种亚致死剂量的杀虫剂均可抑制斑翅果蝇的MFO的活性,而对黑腹果蝇的MFO活性具有明显的促进作用。由此可见,在亚致死剂量杀虫剂的处理下,两种果蝇体内的解毒酶活性均会发生变化,但应激反应并不相同,这为两种果蝇抗药性以及综合防治的差异分析提供了重要的指导作用。

To clarify the effects of emamectin, spinetoram and beta-cyfluthrinon with sublethal concentration on detoxification enzymes in Drosophila melanogaster and D. suzukii, the activities of detoxification enzymes were measured,such as acetylcholin esterase（AchE）,glutathione transferase（GSTs）,carboxylesterase（CarE） and multifunctional oxidase（MFO）. The results showed that the activity of Ach E and GSTs in D. suzukii adult was activated by emamectin,while inhibited in D.melanogaster. The activity of Car E in two Drosophila speceis was activated by spinetoram and beta-cyfluthrinon. However,GSTs activity in D. melanogaster was significantly inhibited by spinetoram and beta-cyfluthrinon with the concentration of LC20. Moreover, these three pesticides with sublethal concentrations activated the activity of MFO in D. melanogaster,while inhibited that in D. suzukii. It was concluded that sublethal concentrations of pesticides could affect the activities of detoxification enzymes differently for D. melanogaster and D. suzukii,which will be guide for the research on the difference of pesticide-resistance and control strategy for D. melanogaster and D. Suzukii.