蒲公英总黄酮含量测定及对小鼠体液免疫功能的影响

Determination of total flavonoids content and effects of Taraxacum mongolicum Hand. Mazz. on humoral immune function in mice

目的研究不同采集时间蒲公英(根、叶、花)总黄酮含量变化规律及含量最高时期蒲公英根、叶、花总黄酮对小鼠体液免疫功能的影响。方法超声提取法提取蒲公英中总黄酮,用HPD-400型大孔树脂进行纯化,紫外分光光度法测定总黄酮含量;溶血素测定法测定小鼠体液免疫功能。结果蒲公英总黄酮含量4月10日采集的最高。同一时期蒲公英根、叶、花中总黄酮含量高低为花>叶>根。蒲公英根、叶、花总黄酮均有增强小鼠体液免疫的作用,其中花作用最强,与黄芪精口服液组相比,差异无统计学意义(P>0.05)。结论不同时期蒲公英根、叶、花中总黄酮含量不同,4月10日采集的蒲公英花含量最高;蒲公英花总黄酮增强小鼠体液免疫的作用最强。

Objective To study the changes of total flavonoids in different stages of Taraxacum mongolicum Hand.Mazz.（root,leaf,flower） and the effects of total flavonoids on the humoral immune function in mice.Methods Total flavonoid was extracted by ultrasonic extraction method and purified by HPD-400 macroporous resin.Ultraviolet spectrophotometric method was used for the determination of the content of total flavones of T.mongolicum Hand.Mazz.in different periods,and hemolysin assay method was used for determination of humoral immune function.Results The content of total flavonoids of root,leaf and flower from T.mongolicum Hand.Mazz.were different in different periods.In the same period,the content of total flavonoids from high to low was flower 〉 leaf 〉 root.The total flavonoids of root,leaf and flower from T.mongolicum Hand.Mazz.could enhance humoral immune effect （compared with negative control group,P〈0.05）.The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice.Compared with Astragalus essence oral liquid there was not statistically significant （P〉0.05）.Conclusion The content of total flavonoids of flower from T.mongolicum Hand.Mazz.was collected in April 10 which was much higher than others.The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice.