一株耐盐植物内生真菌的筛选与鉴定

The Screening and Identification on an Endophytic Fungi of Halotolerant

[目的]研究盐田原生态系统中的耐盐植物内生真菌。[方法]对38株从海南省莺歌海盐场和东方盐场分离的菌株进行耐盐生长测试。[结果]发现了1株在8%Na Cl浓度下生长较好的真菌,并根据其形态特征,ITS扩增序列聚类进化树分析,显示其分类地位为黑酵母菌(Hortaea werneckii)。经过高效液相色谱分析其代谢产物,发现该真菌在0、2%、4%、8%Na Cl浓度下的发酵产物均有所不同。[结论]盐田生境中存在特殊的植物内生真菌区系,其中黑酵母菌Hortaea werneckii具有一定代表性,有深入研究的价值。

[Objective]To study the endophytic fungi of halotolerant in the original ecosystem of saltern. [Method]Salt stress tests were conducted on the endophytic fungi isolated from Yingehai Saltern and Dongfang Saltern in Hainan Province,to discover the halotolerant fungi among them. [Result]One isolate was further identified according to morphological and ITS sequence-based phylogenic analyses,which suggested it belongs to Hortaea werneckii,a black yeast species,in taxonomy. The ferment substance under 0,2%,4%,8% of sodium chloride of this isolate was studied with HPLC finger printing approach,and differential metabolites according to salt concentrations were observed. [Conclusion]The solar salterns may be occupied by idiographic microflora of endophytes,included Hortaea werneckii observed in this study,which maybe representative of further studies.