组培柠檬桉叶绿素和挥发油研究

Study on Chlorophyll and Essential Oil Content of Eucalyptus citriodora

[目的]研究柠檬桉叶绿素和挥发油含量。[方法]采用分光光度法测定柠檬桉叶绿素含量,对比不同提取液对柠檬桉叶绿素提取效果,采集不同季节柠檬桉,采用传统水蒸气蒸馏法对挥发油含量以及成分进行分析。[结果]丙酮∶乙醇=1∶1(V/V)混合提取液对柠檬桉叶绿素的提取效果较佳,叶绿素a/叶绿素b为3.580。季节对组培柠檬桉挥发油得率有一定的影响,10月份采集柠檬桉挥发油含量相对较高,绝干得油率为3.526%,1月份最低,仅为2.880%(以绝干计)。[结论]组培柠檬桉的生长情况较好,但挥发油含量总体偏低,建议进行合理管理,有效提高柠檬桉鲜叶挥发油含量。

[Objective]To study chlorophyll and volatile oil content of Eucalyptus citriodora,respectively. [Method]The chlorophyll content of Eucalyptus citriodora was determined by UV spectrophotometry,and the effect of different extract-solution on chlorophyll extraction was compared.Eucalyptus citriodora oil from fresh leaves in different seasons was obtained by steam distillation,and the chemical compositions of Eucalyptus citriodora oil were analyzed by gas chromatography-mass spectrometry（ CG-MS）.[Result]Acetone ∶ethanol = 1 ∶1（ V/V） mixed extract had a better effect on the extraction of chlorophyll from Eucalyptus citriodora. The ratio of chlorophyll a/chlorophyll b was 3. 580. Moreover,the essential oil yield of Eucalyptus citriodora showed certain difference in different seasons,the relatively higher in October（ 3. 526%） and the lowest in January（ 2. 880%）.[Conclusion]The growth situation of Eucalyptus citriodora with tissue culture was good,but the content of volatile oil was generally low. Thus,it was suggested that reasonable management could improve the quality of Eucalyptus citriodora.