巴戟天低聚糖改善不育症小鼠生殖能力的作用研究

Study on Oligosaccharide Extracted from Morinda officinalis Improve the Reproductive Ability of Mice

目的探讨巴戟天低聚糖(oligosaccharide extracted from Morinda officinalis,OMO)对小鼠生殖能力的保护作用。方法实验分为5组,对照组(蒸馏水)、模型组(肾阳虚组)、巴戟天低聚糖(OMO)低、中、高剂量治疗组(分别含药0.02g、0.04g、0.08g,灌胃给药)。采用"劳倦过度、房事不节"法复制小鼠肾阳虚模型。给药6周后,观察生殖激素水平及丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量。结果肾阳虚小鼠灌胃OMO中、高剂量治疗后血促卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)、促性腺激素释放激素(Gn RH)均明显升高(P<0.01),小鼠睾丸组织匀浆MDA浓度明显降低,而SOD、GSH-Px浓度明显升高(P<0.01)。结论 OMO可能通过调节垂体-下丘脑-性腺轴的功能来调节生精过程,并且增强体内抗氧化酶活性,增强睾丸功能,从而发挥抗氧化作用,保护睾丸精子免受氧化损伤。

Objective To explore the effect of oligosaccharides of Morinda officinalis to protect the reproductive ability of mice. Methods The test were randomly divided into five groups, control group（ constant volume distilled water）,model group（ deficiency of kidney yang）, oligosaccharide extracted from Morinda officinalis high-dose, medium-dose and low-dose groups group（ the dosage of OMO was 0.02 g、 0.04 g、 0.08 g, respectively） with intragastrical administration. The mice model was induced by tired excessive sex not section method. After 6 weeks, the reproductive hormone levels, MDA,SOD and GSH-Px in mice were detected. Results The middle and high does of oligosaccharide extracted from Morinda officinalis group was compared to the model group as follows ：the containments of FSH, LH, T and Gn RH in the blood became higher（ P〈0.01）, containment of MDA in the epididymis tissue was lower, and concentrations of SOD and GSH-Px increased（ P〈0.01）. Conclusion The oligosaccharides of Morinda officinalis might regulate the function of spermatogenic process by regulating the pituitary-hypothalamus-gonadal axis, and enhance the body antioxidant activity and epididymis,and thus play a role in the protection of epididymal sperm from oxidative damage.