基于“情志致病”理论建立慢性咽炎肝郁脾虚大鼠模型的研究

Study on the Type of Liver-stagnation and Spleen-deficiency Chronic Pharyngitis Rat Model that Based Emotion Pathopoiesis Theory

目的通过建立慢性咽炎肝郁脾虚大鼠模型,探讨慢性咽炎的发生发展与影响情绪的关键神经递质含量的相关性,从而研究慢性咽炎与"情志致病"的中医致病理论之间的关系。方法选用70只Wistar大鼠构建慢性咽炎模型,随机分7组:空白对照组,标准慢性咽炎模型组,标准慢性咽炎模型低剂量组,标准慢性咽炎模型高剂量组,慢性咽炎肝郁脾虚模型组,慢性咽炎肝郁脾虚低剂量组,慢性咽炎肝郁脾虚高剂量组,造模完成并评估后分别给予"咽炎消合剂"治疗10 d,检测各组大鼠下丘脑组织5HT-m RNA表达,并对各组大鼠行咽黏膜病理切片检测。结果 2种模型大鼠下丘脑组织5HT-m RNA表达低于空白组,肝郁脾虚组的5-HT2C含量明显低于标准模型组,5-HT1B高于标准模型组。经予"咽炎消合剂"治疗后,5-HT1B、5-HT2C受体增加。病理切片显示与2种模型对比治疗前,其咽黏膜慢性炎细胞的数量减少,咽部血管通透性及炎细胞渗出减轻。结论慢性咽炎肝郁脾虚大鼠模型建立成功,比标准模型更合乎临床病理特征,证实大鼠体内慢性咽炎的发生发展与影响情绪的关键物质神经递质的异常表达有直接的关联,证明了慢性咽炎中医致病理论"情志致病"的正确性。

Objective To confirm the theory of emotion pathopoiesis that basis of TCM, through establishing a rat model of chronic pharyngitis（ CP） liver-stagnation and spleen-deficiency CP rat model（ GYPXM） to explore the correlation between the occurrence and development of CP and the expression of the key neurotransmitter. Methods 70 wistar rats were used to establishing the CP standard model and liver-stagnation and spleen-deficiency CP rat model, that divided into 7 groups randomly. After modeling, the hypothalamus tissue 5 HT m RNA expression in each group was examined, and pathological sections of rats in each group were observed. Results The hypothalamus 5 HT m RNA expression in both models was lower than that in the blank group. After 10 days of Yanyanxiao mixture treatment, the 5-HT1 B, 5-HT2 C receptor two kind of rat model were increased. Conclusion Compared with the standard model, CP rat model of liver-spleen deficiency established successfully, and is more suitable for clinical and pathological features. The incidence of CP is associated with abnormal expression of the key neurotransmitter which affectded by mood.