电解质水对粪肠球菌生物膜杀菌机制的初步探索

The preliminary exploration of bactericidal mechanism of electrolyzed water on Enterococcus faecalis biofilm

目的:研究强酸性电解质水(SAEW)和碱性电解质水(AEW)对粪肠球菌生物膜的作用。方法:体外培养粪肠球菌生物膜,分为SAEW处理组、AEW处理组及52.5g/L次氯酸钠(NaClO)处理组,各组分别处理0、1、2、3、4、5、10、15、30、60min后,采用菌落计数法测定各冲洗液对粪肠球菌生物膜的杀菌率;另取培养24h的粪肠球菌生物膜,分别用SAEW、AEW、52.5g/L NaClO、生理盐水处理1、5、10min后,用扫描电镜观察粪肠球菌生物膜的形态变化。结果:SAEW和52.5g/L NaClO对粪肠球菌各时间点的杀菌率显著高于AEW(P<0.05),而SAEW和52.5g/L NaClO的杀菌率无统计学差异(P>0.05);扫描电镜观察结果显示,随着处理时间的延长,SAEW、AEW及52.5g/L NaClO处理后粪肠球菌的形态变化类似,均表现为生物膜中粪肠球菌减少,细菌表面粗糙且菌体开始皱缩。结论:电解质水对粪肠球菌生物膜中的菌株具有杀灭作用,且其作用机制类似于NaClO。

AIM： To study the bactericidal efficiency and possible bactericidal mechanism of strong acid electrolyzed water （SAEW） and alkaline electrolyzed water （AEW）on Enterococcus faecalis （ E.faecalis ） biofilm. METHODS： The E.faecalis biofilm on coverslips was cultured and treated by 52.5 g/L NaClO（control）, SAEW and AEW respectively.The effects were examined by the viable cell count method and the morphological change of E.faecalis was observed by scanning electron microscopy. RESULTS： The bactericidal efficiency of SAEW and 52.5 g/L NaClO showed no significant difference（ P 〉0.05）. SAEW showed greater bacterial reduction than AEW（ P 〈0.05）. But after treatment, the trend of the time-dependent curves and the morphological change were similar among the 3 preparations . CONCLUSION： Both SAEW and AEW had bactericidal efficiency to E.faecalis . And the bactericidal mechanism was similar to that of NaClO.