钛种植体表面阳极氧化及其生物学性能的研究

Influence of Anodic Oxidation Modification on Biological Properties of Titanium Implant

目的:在钛种植体表面进行阳极氧化后,研究其表面微形貌及生物学性能。方法:通过阳极氧化在光滑纯钛表面形成TiO_2纳米管,用扫描电镜、接触角测定仪对光滑纯钛及TiO_2纳米管进行检测。在阳极氧化后的钛表面接种MC3T3-E1成骨细胞,观察其细胞的黏附与增殖情况并对其生物学性能进行检测。结果:(1)阳极氧化后TiO_2纳米管试样表面形成了比较均匀、呈垂直状排列的管状结构,管径大致为70 nm,管壁厚度约为10 nm,管间隙约为10 nm;(2)TiO_2纳米管组亲水性大于纯钛组。(3)TiO_2组比Ti组具有更快的细胞增殖速度,培养到第2天时,TiO_2试样表面的细胞数目明显多于纯Ti组(P<0.05)。(4)TiO_2组表面覆盖生长更多的成骨细胞、而且细胞表面积更大,扩展更充分。结论:通过阳极氧化处理方法可以在纯钛表面得到有序的纳米管状结构,而且该结构能使成骨细胞在早期就表现出更快的增殖速度请补充。

Objective： To explore surface features and biological properties of titanium implant after anodic oxidation modification. Methods： Pure titanium was anodized to obtain TiO2 nanotube. Thereafter the pure titanium and TiO2 nanotube were characterized by scanning electron microscopy and contact angle instrument. The attachment and spreading of MC3 T3-E1 osteoblasts on TiO2 nanotube were also investigated. Results：（1）By anodic oxidation it was possible to fabricate regularly and vertical TiO2 nanotube,the diameter was about 70 nm,the thickness of tube was about 10 nm,the distance between tubes was about 10 nm;（2）The hydrophily of TiO2 nanotube was bigger than pure titanium implant;（3）The cell proliferation rate of TiO2 nanotube was faster than that of pure titanium implant,when it came to the 2 nd day,the number of cells on TiO2 nanotube obviously larger than pure titanium implant（P〈0. 05）;（4） The surface of TiO2 nanotubes were covered with bigger quantity and superficial area of osteoblasts. Conclusion： By anodic oxidation modification,we could construct orderly TiO2 nanotube on the surface of pure titanium implant,and the osteoblasts were showed faster proliferation rate in the early stage.