表面分子印迹材料的制备及在生物分子分离分析中的应用

Preparation of Surface Molecularly Imprinted Polymers and Its Application in Biomolecular Separation and Recognition

表面分子印迹技术是一种新型的分子印迹技术,其解决了传统印迹方法得到的印迹聚合物模板结合位点少、洗脱困难、色谱性能和机械性能差等问题。表面分子印迹聚合物(SMIPs)以其稳定性好、特异性高及实用性强的特点,近年来在生物分子分离分析中的应用引起人们的关注。本文介绍了SMIPs的制备方法,比较了不同方法的优缺点,并对其在生物大分子、生物小分子及微生物分子分离分析中的应用进行了综述。

Surface molecular imprinting technology is a new type of molecular imprinting technology. It solves the problems of difficulty in incomplete template removal,low binding capacity,poor chromatographic performance and poor mechanical properties. Surface molecular imprinting polymers have attracted much attention due to their outstanding advantages,such as good chemical stability,high specificity and strong practicality. In recent years,it has been used widely in the separation and analysis of biochemical samples. This article describes the preparation of surface molecularly imprinted polymers, compares the advantages and disadvantages of different methods, and summarizes the application of surface molecular imprint in the separation and analysis.