不同食物对肠旁路术后2型糖尿病鼠血糖血脂的影响

The effect of different food influence on glucose and blood lipids in type Ⅱ diabetic model rats after duodenal-jejunal bypass surgery

目的观察不同食物对十二指肠旁路术（DJB）及改良胆胰转流术（NBPD）后2型糖尿病模型鼠血糖及血脂的影响，探讨手术治疗2型糖尿病的机制。方法将33只结合高脂高糖饮食和小剂量（35 mg/kg）链脲霉素腹腔注射造模成功的2型糖尿病大鼠随机分为3组，分别行十二指肠旁路术、改良胆胰转流术及假手术。检测术前1周、术后1、4、10周大鼠空腹体重、血糖及餐后2 h血糖变化。术后3～5周分别以脂肪乳、花生油、安素、葡萄糖、淀粉灌胃，检测大鼠血脂、血糖变化。结果术后1周，DJB组大鼠餐后2 h血糖降至（19.87±4.07） mmol/L，NBPB组降至（20.34±5.76） mmol/L，与假手术组比较，差异有统计学意义（P=0.001）。术后10周，DJB组餐后2 h血糖（17.15±3.75） mmol/L，NBPD组餐后2 h血糖（20.16±4.73） mol/L，与假手术组比较，差异有统计学意义（P=0.001）。术后4周脂肪乳灌胃后DJB组大鼠游离脂肪酸升至（1 082.83±259.67） μEq/L，NBPD组升至（1 258.67±204.18） μEq/L，与假手术组的（1 866.00±715.15） μEq/L比较，差异有统计学意义（P=0.000）。结论DJB和NBPD术后餐后血糖、血脂降低，其机制可能是术后消化吸收功能改变。

ObjectiveThe aim of this study was to investigate the influence of glucose and blood lipids with different food after duodenal-jejunal bypass （DJB） and new biliopancreatic diversion surgery （NBPD）on Diabetic model rats.MethodsThirty-three diabetic model rats were randomly assigned to the DJB group （n=12）, NBPD group （n=11）, and sham group （n=10）. Body weight, glucose, blood lipids after mixed-meal were measured 1 week before surgery and 1, 4, 10 weeks after surgery.3-5 weeks after surgery, after Peanut oil, fat milk, Ansul, glucose and starch was administered by oral gavage at a dose of 8 kcal/kg body weight, blood lipids and glucose were measured.Results2 hours postprandial glucose of DJB group was decreased to （19.87±4.07） mmol/L 1 week after surgery, while （20.34±5.76） mmol/L in NBPD group, it was lower than what in sham group （P=0.001）.10 weeks after surgery, 2 hours postprandial glucose of DJB group was （17.15±3.75） mmol/L, while （20.16±4.73） mol/L in NBPD group （P=0.001）.4 weeks after surgery, FFA was raised from （534.60±70.99） μEq/L to （1 082.83±259.67） μEq/L after fat milk fed in DJB group, while from （648.33±139.26） μEq/L to （1 258.67±204.18） μEq/L in NBPD group, and it was lower than what in sham operation group’s which is （1 866.00±715.15） μEq/L （P=0.000）.ConclusionThe postprandial glucose and blood lipids after DJB and NBPD was decreased, the mechanism may be the change of the function of digestion and absorption.