利胆消黄汤对大鼠肝脏缺血再灌注氧化应激的保护作用

Protective effect of Lidan Xiaohuang decoction on hepatic ischemia reperfusion oxidative stress in rats

目的观察利胆消黄汤对大鼠肝脏缺血再灌注损伤的保护作用。方法将225只SD大鼠随机分为假手术组（Sham组）、缺血再灌注损伤（HIRI）对照组（Control组）、HIRI利胆消黄汤组（TCM组），每组按再灌注后60、180、360 min分为3个时相。TCM组大鼠术前予中药灌胃，Control组及Sham组给予等量生理盐水灌胃；Sham组仅进行开关腹操作，TCM组和Control组进行HIRI处理。建立大鼠HIRI模型，于肝脏缺血再灌注60、180、360 min时从下腔静脉取血，测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）含量，同时留取新鲜肝脏标本，测定组织丙二醛（MDA）含量、谷胱甘肽过氧化物酶（GSH-PX）活力，并进行组织病理学观察。结果大鼠HIRI分别60、180、360 min后，TCM组中ALT、AST和LDH水平分别为（74.52±8.95） U/L、（89.48±16.87） U/L、（118.95±18.47） U/L，（225.94±49.76） U/L、（276.36±78.95） U/L、（329.85±79.59） U/L和（1 467.44±295.46） U/L、（2 860.59±347.32） U/L、（3 020.22±357.83） U/L。MDA含量和GSH-PX活力分别为（3.94±0.86） nmol/mg蛋白质、（7.33±3.12） nmol/mg蛋白质、（12.89±6.48） nmol/mg蛋白质和（1866.34±364.49） U/mg蛋白质、（1299.05±269.49） U/mg蛋白质、（780.77±185.03） U/mg蛋白质；Control组中的ALT、AST和LDH水平分别为（99.24±12.71） U/L、（182.37±21.62） U/L、（298.13±36.26） U/L，（356.87±57.36） U/L、（529.31±79.39） U/L、（875.69±83.61） U/L和（1937.59±279.55） U/L、（4207.43±654.87） U/L、（4 672.66±539.11） U/L。MDA含量和GSH-PX活力分别为（6.76±1.56） nmol/mg蛋白质、（14.20±4.87）nmol/mg蛋白质、（25.82±9.86） nmol/mg蛋白质和（1689.33±339.73） U/mg蛋白质、（792.19±241.66） U/mg蛋白质、（429.80±126.07） U/mg蛋白质。与Control组比较，TCM组中ALT、AST、LDH、MDA的含量均明显较低，GSH-PX活性下降幅度较小，差异均有统计学意义（P=0.000）；Sham组各时相血清ALT、AST、LDH水平、MDA含量及GSH-PX活力变化幅度较小（P=0.000）。病理学观察，TCM组和Control组也表现出明显的差异性。结论利胆消黄汤能够对大鼠HIRI具有保护作用，保护机制与其抗氧化机制有关。

ObjectiveTo observe the protective effect of Lidan Xiaohuang decoction on hepatic ischemia reperfusion injury in rats.Methods225 SD rats were randomly divided into sham-operated group （Sham group）, ischemia reperfusion injury （HIRI） control group （control group） and HIRI traditional Chinese medicine group （TCM group）. Each group was divided into three subgroups at 60, 180 and 360 min after reperfusion. Rats in TCM group were given Chinese medicine gavage before operation. Control group and Sham group were given the same volume of normal saline. In Sham group, only open abdominal operation was performed, and TCM group and control group were treated with HIRI. A rat HIRI model was established and blood was collected from the inferior vena cava at 60, 180 and 360 min after ischemia and reperfusion in the liver. Alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and lactate dehydrogenase （LDH） levels were measured, and fresh liver specimens were taken at the same time for measuring MDA content and GSH-PX activity, and histopathological observations were performed.ResultsAfter 60, 180 and 360 min of HIR in rats, the ALT, AST and LDH in TCM group were （74.52±8.95）, （89.48±16.87）, （118.95±18.47）, （225.94±49.76）, （276.36±78.95）, （329.85±79.59）, （1 467.44±295.46）, （2 860.59±347.32）, （3 020.22±357.83） U/L respectively. The MDA content and the activities of GSH-PX were respectively （3.94±0.86）, （7.33±3.12）, （12.89±6.48） nmol/mgprot and （1 866.34±364.49）, （1 299.05±269.49）, （780.77±185.03） U/mgprot; The ALT, AST and LDH levels in the control group were respectively （99.24±12.71）, （182.37±21.62）, （298.13±36.26）, （356.87±57.36）, （529.31±79.39）, （875.69±83.61）, （1 937.59±279.55）, （4 207.43±654.87）, （4 672.66±539.11） U/L. And the MDA content and the activity of GSH-PX were （6.76±1.56）, （14.20±4.87）, （25.82±9.86） nmol/mgprot and （1 689.33±339.73）, （792.19±241.66）, （429.80±126.07） U/mgprotrespectively. As compared with the control group, the levels of ALT, AST, LDH and MDA contents in the TCM group were significantly reduced, and the GSH-PX activity was slightly decreased （P=0.000）; The changes of ALT, AST and LDH levels, MDA content and GSH-PX activity in the Sham group were smaller （P=0.000）. Pathological observation showed that TCM group and Control group also had a significant difference.ConclusionLidan Xiaohuang decoction can protect against HIRI in rats, and the protective mechanism is related to its antioxidant mechanism.