肝内胆管细胞癌中K-ras基因突变分析及临床意义

Analysis and significance of K-ras gene mutation in intrahepatic cholangiocarcinoma

目的检测肝内胆管细胞癌组织K-ras基因突变状况，探讨K-ras基因突变与肝内胆管细胞癌的关系。方法提取32例肝内胆管细胞癌及12例肝内胆管良性病变石蜡组织切片中基因组DNA，PCR扩增K-ras第2外显子片段，采用直接测序法检测其突变状况。结果32例肝内胆管细胞癌患者中有24例患者存在K-ras基因突变，12例良性病变组织均未发现突变，两者差异具有统计学意义（χ^2=14.570，P=0.000）。其中22例12密码子突变（91.7%），1例6密码子突变，1例23密码子突变。结论K-ras基因突变与肝内胆管细胞癌的发生明显相关。

ObjectiveTo detect the K-ras gene mutation in intrahepatic cholangiocarcinoma by direct sequencing, and explore the relationship between K-ras gene mutation and pathogenesis of intrahepatic cholangiocarcinoma.MethodsThe genomic DNA was extracted from paraffin tissue sections of 32 cases of intrahepatic cholangiocarcinoma and 12 cases of intrahepatic bile duct benign lesions, respectively. K-ras 2 exon fragments were amplificed by PCR and their mutation status was detected by direct sequencing.Results24 cases were found The K-ras gene mutation was found in 24 out of 32 patients with intrahepatic cholangiocarcinoma, and no K-ras gene mutation was detected in 12 benign lesions with the difference being significant （χ^2=14.570, P=0.000）. There were 22 cases with codon 12 mutation （91.7%）, 1 case with codon 61 mutation, and 1 case with codon 23 mutation.ConclusionThe mutation of the K-ras gene may be closely related to the pathogenesis of intrahepatic cholangiocarcinoma.